Research Unit on Women's Health-Institute of Health Research INCLIVA, 46010 Valencia, Spain.
Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, 46010 Valencia, Spain.
Int J Mol Sci. 2018 Jun 22;19(7):1848. doi: 10.3390/ijms19071848.
The system integrated by the receptor activator of nuclear factor kappa B (RANK) and its ligand, RANKL, modulates the role of hormones in the genesis and progression of breast tumors. We investigated whether the expression of RANK was related with clinicopathological features of primary endometrial tumors. Immunohistochemistry was used in an endometrial cancer tissue array containing samples from 36 tumors. The amount of RANK mRNA was examined in a tissue scan cDNA array containing cDNA from 40 tumors. Normal endometrium was examined for comparison. Immunohistochemical analyses showed that RANK expression was higher in malignant than in normal endometrium ( < 0.05). RANK expression was related to histological grade (Pearson correlation index = 0.484, < 0.001), but not to tumor stage or to age of the women. The gene expression was similar in malignant and normal endometrium. The study of RANK isoforms confirmed that the overall relative abundance of the three clearly identified transcripts was similar in normal and pathological endometrium. RANK protein expression increased from normal to malignant endometrium, and the expression level was related with tumor grade but not with stage or the age of subjects in endometrial cancer. In contrast, similar comparisons showed no change in RANK gene expression.
核因子 κB 受体激活剂（RANK）及其配体 RANKL 构成的系统调节着激素在乳腺癌发生和发展中的作用。我们研究了 RANK 的表达是否与原发性子宫内膜肿瘤的临床病理特征有关。应用免疫组织化学方法检测了包含 36 例肿瘤样本的子宫内膜癌组织芯片中的 RANK 表达。采用组织扫描 cDNA 芯片检测了包含 40 例肿瘤 cDNA 的 RANK mRNA 量。同时检测了正常子宫内膜作为对照。免疫组化分析显示 RANK 在恶性肿瘤中的表达高于正常子宫内膜（<0.05）。RANK 的表达与组织学分级相关（皮尔逊相关指数=0.484，<0.001），但与肿瘤分期或患者年龄无关。在恶性和正常子宫内膜中基因表达相似。对 RANK 异构体的研究证实，在正常和病理性子宫内膜中，三种明确鉴定的转录本的总相对丰度相似。RANK 蛋白表达从正常子宫内膜增加到恶性子宫内膜，其表达水平与肿瘤分级相关，但与肿瘤分期或患者年龄无关。相比之下，相似的比较显示 RANK 基因表达没有变化。
