Department of Physics, Photonics Lab, University of Kharazmi, Karaj, Iran; Biophotonics Lab, Applied Science Research Center (ASRC), Kharazmi University, Karaj, Iran.
ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran; Medical Physics Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Photodiagnosis Photodyn Ther. 2018 Sep;23:171-175. doi: 10.1016/j.pdpdt.2018.06.015. Epub 2018 Jun 19.
This study aimed to explore the potential of the Z-scan technique to improve accuracy in identifying SK-BR-3 and MCF-7 breast cancer cell lines.
Three in vitro samples were prepared for each breast cancer cell line. A closed-aperture Z-scan technique was used to measure the sign and magnitude of the nonlinear refractive index of each sample. Prior to the Z-scan, all samples were fixed with 1 mL of 5% paraformaldehyde.
The sign of the nonlinear refractive indices of MCF-7 and SK-BR-3 breast cancer cell lines were negative and positive, respectively. The repeated Z-scan measurements for all samples of each cell line were similar.
The results indicated that the proposed bio-optical method is a reliable method for characterizing differences in various breast cancer cell types. It is suggested that the nonlinear refractive index of cells be considered as an indicator for differentiating various breast cell lines from each other.
本研究旨在探索 Z 扫描技术在提高识别 SK-BR-3 和 MCF-7 乳腺癌细胞系准确性方面的潜力。
为每种乳腺癌细胞系制备了三个体外样本。采用闭孔径 Z 扫描技术测量每个样本的非线性折射率的符号和大小。在 Z 扫描之前，所有样本均用 1ml5%的多聚甲醛固定。
MCF-7 和 SK-BR-3 乳腺癌细胞系的非线性折射率的符号分别为负和正。每个细胞系的所有样本的重复 Z 扫描测量结果相似。
结果表明，所提出的生物光学方法是一种可靠的方法，可用于表征不同乳腺癌细胞类型的差异。建议将细胞的非线性折射率作为区分不同乳腺癌细胞系的指标。
