Wheeler M E, Gerrard J M, Carroll R C
J Cell Biol. 1985 Sep;101(3):993-1000. doi: 10.1083/jcb.101.3.993.
Concanavalin A (Con A) has been used to activate platelets, inducing a specific interaction between the glycoprotein IIb-IIIa complex and the cytoskeleton of the activated platelet. In agreement with this, we have shown that Con A activates human platelets, initiating phosphorylation, secretion, and cytoskeletal formation. Con A and cytochalasin B were used to demonstrate a reciprocal interaction of the glycoprotein complex with the platelet cytoskeleton. Additionally, we have shown that a similar reciprocity is provided by the multivalent fibrin-fibrinogen platelet interaction found in the thrombin-induced clot. Con A differs from other activators in precipitating an apparent cytoskeletal core despite a complete inhibition of platelet activation by prostaglandin E1. We suggest, from this result, that Con A may be cross-linking a membrane-associated cytoskeletal complex present in the unactivated platelet.
伴刀豆球蛋白A（Con A）已被用于激活血小板，诱导糖蛋白IIb-IIIa复合物与活化血小板的细胞骨架之间发生特异性相互作用。与此一致的是，我们已经表明Con A可激活人血小板，引发磷酸化、分泌及细胞骨架形成。Con A和细胞松弛素B被用于证明糖蛋白复合物与血小板细胞骨架之间的相互作用。此外，我们已经表明，在凝血酶诱导的凝块中发现的多价纤维蛋白-纤维蛋白原血小板相互作用也提供了类似的相互作用。尽管前列腺素E1完全抑制了血小板活化，但Con A与其他激活剂不同，它能沉淀出一个明显的细胞骨架核心。基于这一结果，我们认为Con A可能正在交联未活化血小板中存在的膜相关细胞骨架复合物。
