Detection of sendai virus protein by reversed-phase high-performance liquid chromatography combined with immuno-chromatography.

Reversed-phase high-performance liquid chromatography (RP-HPLC) was applied to the detection of Sendai virus F2 protein. Immunoaffinity chromatography was used prior to RP-HPLC to increase the specificity. With this combined method, the presence of F2 protein in complex solutions could be demonstrated within 150 min. Using RP-HPLC, F2 protein could be detected in amounts down to 20 ng, which could be reduced to 1 ng by application of a microbore column.