在Triton X-100存在的情况下，通过阴离子交换高效液相色谱法分离仙台病毒F蛋白。
Isolation of Sendai virus F protein by anion-exchange high-performance liquid chromatography in the presence of Triton X-100.
作者信息
Welling G W, Groen G, Welling-Wester S
出版信息
J Chromatogr. 1983 Aug 26;266:629-32. doi: 10.1016/s0021-9673(01)90932-x.
PMID:6313723
Abstract
Purified Sendai virions were treated with Triton X-100. The detergent extract containing the fusion protein (F) and the haemagglutinin-neuraminidase protein (HN) was subjected to anion-exchange high-performance liquid chromatography on a Mono Q (Pharmacia) column with 0.1% Triton X-100 in phosphate-buffered saline. HN was not retained by the column while elution with a salt gradient resulted in several peaks containing mainly or only F protein.
摘要
纯化的仙台病毒粒子用Triton X-100处理。含有融合蛋白（F）和血凝素神经氨酸酶蛋白（HN）的去污剂提取物在含有0.1% Triton X-100的磷酸盐缓冲盐水中，通过Mono Q（Pharmacia）柱进行阴离子交换高效液相色谱分析。HN不被柱子保留，而用盐梯度洗脱则产生几个主要或仅含F蛋白的峰。
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