超高效液相色谱法同时测定小儿豉翘清热颗粒中9种成分
[Simultaneous determination of 9 components in Xiao'er Chiqiao Qingre granules by UPLC].
作者信息
Tian Gang, Yang Xue-Fang, Li Chao, Wu Fei, Liu Xue, Feng Liang, Jia Xiao-Bin
机构信息
Jumpcan Pharmaceutical Co., Ltd., Taizhou 225411, China.
Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2081-2085. doi: 10.19540/j.cnki.cjcmm.20180208.005.
To establish a method for the simultaneous determination of 9 components, namely gardenoside, paeoniflorin, forsythoside A, baicalin, forsythin, glycyrrhizic acid, rhein, honokiol, and magnolol in Xiao'er Chiqiao Qingre granules（XECQ Gra）. Ultra performance liquid chromatography (UPLC) was used on an Acquity UPLC HSS T3 C₁₈ column (2.1 mm×100 mm, 1.8 μm) with 0.1% phosphoric acid acetonitrile (A)-0.1% phosphoric acid solution (B) as mobile phase for gradient elution. The flow rate was 0.3 mL·min⁻¹ ; the column temperature was set at 30 °C, and the determination wavelength was set at 220 nm. All the 9 compounds were well separated, and showed good linear relationship within their concentrations (r>=0.999). The average recoveries were between 95.84%-101.4% and the RSD values were all less than 3.0%. The method is simple, reliable, and accurate, and could be used for the quality control of XECQ Gra.
建立同时测定小儿豉翘清热颗粒中9种成分（栀子苷、芍药苷、连翘酯苷A、黄芩苷、连翘苷、甘草酸、大黄酸、和厚朴酚、厚朴酚）的方法。采用超高效液相色谱法（UPLC），以Acquity UPLC HSS T3 C₁₈色谱柱（2.1 mm×100 mm，1.8 μm），以0.1%磷酸乙腈（A）-0.1%磷酸溶液（B）为流动相进行梯度洗脱。流速为0.3 mL·min⁻¹；柱温设定为30℃，检测波长设定为220 nm。9种化合物均得到良好分离，在其浓度范围内呈现良好的线性关系（r>=0.999）。平均回收率在95.84%-101.4%之间，相对标准偏差（RSD）值均小于3.0%。该方法简便、可靠、准确，可用于小儿豉翘清热颗粒的质量控制。
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