Huang Tao, Wang Ya-Dong, Xue Ming-Ming, Feng Xue, Sun Cai-Xia, Wang An-Si, Xie Shu-Yu, Zhang Meng, Sun Gui-Rong, Li Ming
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Wenhua Road 95, Zhengzhou, 450002, People's Republic of China.
Genes Genomics. 2018 Jul;40(7):687-700. doi: 10.1007/s13258-018-0651-y. Epub 2018 Mar 26.
Reproduction is a complex physiological process that is regulated by multiple genes and pathways. Compared with studies of common livestock, fewer studies of genes related to the fertility of rabbits (Oryctolagus cuniculus) have been reported, and the molecular mechanism of their high productivity is still poorly understood. To identify candidate genes associated with development and prolificacy in rabbits, we analyzed gene expression differences among the ovaries of mature Californian rabbit (LC), and mature (HH) and immature Harbin white rabbit (IH) using digital gene expression technology. We detected 885 and 321 genes that were significantly differentially expressed in comparisons between HH/IH and HH/LC, respectively. The functions of the differentially expressed genes (DEGs) were determined by GO classification and KEGG pathway analysis. The results suggest that most of the DEGs between the mature and immature developmental stages were predominantly associated with DNA replication, cell cycle, and progesterone-mediated oocyte maturation, and most were up-regulated in the IH group compared with the HH group. The DEGs involved in disparate fecundities between HH and LC were associated with reproduction, fructose and mannose metabolism, steroid hormone biosynthesis, and pyruvate metabolism. Our results will contribute to a better understanding of changes in the regulatory network in ovary at different developmental stages and in different fertility of rabbit.
生殖是一个由多个基因和信号通路调控的复杂生理过程。与常见家畜的研究相比，关于家兔（穴兔）繁殖力相关基因的研究报道较少，其高产的分子机制仍知之甚少。为了鉴定与家兔发育和繁殖力相关的候选基因，我们利用数字基因表达技术分析了成年加利福尼亚兔（LC）、成年哈尔滨白兔（HH）和未成年哈尔滨白兔（IH）卵巢之间的基因表达差异。我们分别在HH/IH和HH/LC的比较中检测到885个和321个显著差异表达的基因。通过基因本体（GO）分类和京都基因与基因组百科全书（KEGG）信号通路分析确定了差异表达基因（DEG）的功能。结果表明，成年和未成年发育阶段之间的大多数差异表达基因主要与DNA复制、细胞周期以及孕酮介导的卵母细胞成熟相关，并且与HH组相比，大多数差异表达基因在IH组中上调。HH和LC之间不同繁殖力所涉及的差异表达基因与生殖、果糖和甘露糖代谢、类固醇激素生物合成以及丙酮酸代谢相关。我们的研究结果将有助于更好地理解家兔卵巢在不同发育阶段和不同繁殖力下调控网络的变化。
