State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.
Biotechnol Adv. 2018 Sep-Oct;36(5):1540-1552. doi: 10.1016/j.biotechadv.2018.06.010. Epub 2018 Jun 20.
Amylosucrase (EC 2.4.1.4, ASase), an outstanding sucrose-utilizing transglucosylase in the glycoside hydrolase family 13, can produce glucans with only α-1,4 linkages. Generally, on account of a double-displacement mechanism, ASase can catalyze polymerization, isomerization, and hydrolysis reactions with sucrose as the sole substrate, and has transglycosylation capacity to attach glucose molecules from sucrose to extra glycosyl acceptors. Based on extensive enzymology research, this review presents the characteristics of various ASases, including their microbial metabolism, preparation, and enzymatic properties, and exhibits structure-based strategies in the improvement of activity, specificity, and thermostability. As a vital transglucosylation tool of producing sugars, carbohydrate-based bioactive compounds, and materials, the bioengineering applications of ASases are also systematically summarized.
淀粉蔗糖酶（EC 2.4.1.4，ASase）是糖苷水解酶家族 13 中一种出色的蔗糖利用转葡糖苷酶，能够生成仅含α-1,4 糖苷键的葡聚糖。通常情况下，由于双置换机制，ASase 可以催化聚合、异构化和水解反应，其唯一的底物是蔗糖，并且具有将葡萄糖分子从蔗糖转移到额外糖基受体上的转葡糖苷能力。基于广泛的酶学研究，本综述介绍了各种 ASase 的特性，包括它们的微生物代谢、制备和酶学性质，并展示了基于结构的策略在提高活性、特异性和热稳定性方面的应用。作为生产糖、碳水化合物基生物活性化合物和材料的重要转葡糖苷工具，ASase 的生物工程应用也得到了系统的总结。
