通过淀粉蔗糖酶合成新型高水溶性葛根素葡萄糖苷。
Enzymatic Synthesis of Novel and Highly Soluble Puerarin Glucoside by Amylosucrase.
机构信息
Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, No. 60 Erh-Jen Rd., Sec. 1, Jen-Te District, Tainan 71710, Taiwan.
Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan.
出版信息
Molecules. 2022 Jun 24;27(13):4074. doi: 10.3390/molecules27134074.
Puerarin (daidzein-8--glucoside) is an isoflavone isolated from several leguminous plants of the genus . Puerarin possesses several pharmacological properties; however, the poor solubility of puerarin limits its applications. To resolve this poor solubility, amylosucrase (AS) was used to modify puerarin into more soluble derivatives. The results showed that AS could biotransform puerarin into a novel compound: puerarin-4'---glucoside. The biotransformation reaction was manipulated at different temperatures, pH values, sucrose concentrations, reaction times, and enzyme concentrations. The results showed that the optimal reaction condition was biotransformed by 200 μg/mL AS with 20% (/) sucrose at pH 6 and incubated at 40 °C for 48 h, and the optimal production yield was 35.1%. Puerarin-4'---glucoside showed 129-fold higher solubility than that of puerarin and, thus, could be further applied for pharmacological use in the future.
葛根素（大豆苷元-8--葡萄糖苷）是从几种豆科植物中分离得到的异黄酮。葛根素具有多种药理作用；然而，葛根素的溶解度差限制了其应用。为了解决这个溶解度差的问题，支链淀粉酶（AS）被用于将葛根素修饰成更易溶解的衍生物。结果表明，AS 可以将葛根素转化为一种新型化合物：葛根素-4'---葡萄糖苷。在不同的温度、pH 值、蔗糖浓度、反应时间和酶浓度下对生物转化反应进行了操作。结果表明，在 40°C 下用 200μg/mL 的 AS 和 20%（/）蔗糖在 pH 6 下转化 48 小时是最佳反应条件，最佳产量为 35.1%。葛根素-4'---葡萄糖苷的溶解度比葛根素高 129 倍，因此，将来可能会进一步应用于药理学用途。
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