Li Wen-Chao, Geng Jin-Zhu, Chen Cheng, Qian Lei, Zhang Tao, Liu Jun-Long, Luo Jian-Xun, Gu You-Fang
College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Acta Trop. 2018 Sep;185:385-390. doi: 10.1016/j.actatropica.2018.06.020. Epub 2018 Jun 21.
Three Entamoeba spp. including E. suis, zoonotic E. polecki, and E. histolytica, have been described in pigs to date. However, little is known about the molecular epidemiology of these neglected parasites in pigs globally. In this study we surveyed the occurrence and molecular epidemiology of porcine Entamoeba spp. in pigs in eastern China and evaluated their zoonotic potential. Five hundred fresh fecal samples, collected from seven pig farms in Anhui province, eastern China,were examined for the presence of E. histolytica, E. suis, and E. polecki ST1 and ST3 infections by a combination of nested PCR targeting the small subunit ribosomal DNA gene and subsequent sequencing.The overall occurrence of Entamoeba spp. was 45.8% (229/500). Infection with E. polecki ST1 (38.2%; 191/500) was the most common, followed by E. polecki ST3 (10.0%; 50/500), and E. suis (0.8%; 4/500). No E. histolytica infection was detected. Double infections with E. polecki ST1 and E. suis, and with E. polecki ST1 and ST3 were found in two (0.4%) and 14 (2.8%) samples, respectively. No age predisposition to infection with Entamoeba spp. was observed. PCR and subsequent sequencing confirmed the validity and feasibility of the nested PCR method used in this study in identifying species/subtypes of porcine Entamoeba spp.This is the first report to describe the occurrence and molecular epidemiology of Entamoeba species in pigs in China. The presence of two zoonotic E. polecki subtypes implies that pigs can be reservoirs for human E. polecki infections. More studiess are needed to better understand the transmission and public health significance of porcine Entamoeba spp.
迄今为止，猪体内已发现三种内阿米巴属物种，包括猪内阿米巴、人兽共患的波列基内阿米巴和溶组织内阿米巴。然而，全球范围内关于这些被忽视的猪寄生虫分子流行病学的了解甚少。在本研究中，我们调查了中国东部地区猪体内猪内阿米巴属物种的发生情况和分子流行病学，并评估了它们的人兽共患病潜力。从中国东部安徽省的七个养猪场收集了500份新鲜粪便样本，通过针对小亚基核糖体DNA基因的巢式PCR及后续测序，检测其中溶组织内阿米巴、猪内阿米巴和波列基内阿米巴ST1和ST3感染情况。内阿米巴属物种的总体感染率为45.8%（229/500）。波列基内阿米巴ST1感染最为常见（38.2%；191/500），其次是波列基内阿米巴ST3（10.0%；50/500），猪内阿米巴（0.8%；4/500）。未检测到溶组织内阿米巴感染。分别在两份（0.4%）和14份（2.8%）样本中发现了波列基内阿米巴ST1和猪内阿米巴的双重感染，以及波列基内阿米巴ST1和ST3的双重感染。未观察到内阿米巴属物种感染的年龄易感性。PCR及后续测序证实了本研究中使用的巢式PCR方法在鉴定猪内阿米巴属物种/亚型方面的有效性和可行性。这是首篇描述中国猪体内内阿米巴物种发生情况和分子流行病学的报告。两种人兽共患的波列基内阿米巴亚型的存在表明猪可能是人类波列基内阿米巴感染的储存宿主。需要更多研究以更好地了解猪内阿米巴属物种的传播及其对公共卫生的意义。
