School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China; School of Agriculture and Biology, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China.
School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China; Shanghai Solid Waste Disposal Co., Ltd., No. 2491, Jiazhu Highway, Jiading District, Shanghai 201815, China.
Sci Total Environ. 2018 Dec 1;643:127-133. doi: 10.1016/j.scitotenv.2018.06.185. Epub 2018 Jun 21.
Autotrophic and mixotrophic denitrification, two approaches of biological denitrification, have drawn more and more attention among the techniques to remove nitrogen from the aquatic environment. This study investigated the influence of phosphorus on the denitrification performance and bacterial community structure in the autotrophic and mixotrophic denitrification reactors. The activity test was applied to evaluate the variation of denitrification activity of autotrophic and mixotrophic sludge before and after phosphorus addition. High-throughput sequencing was used to analyze the change of bacterial community structure. The results showed that NO-N removal efficiency of autotrophic and mixotrophic denitrification process increased by 40 and 35%, respectively, after phosphorus addition. The sludge denitrification activity of autotrophic and mixotrophic sludge was enhanced significantly. And phosphorus addition could greatly improve the proportion of denitrifying bacteria in both autotrophic (from 11.83 to 64.31%) and mixotrophic denitrifying sludge (from 13.59 to 45.12%). Overall, phosphorus addition could greatly improve the autotrophic and mixotrophic denitrification ability in the phosphorus deficient surface water.
自养和混合营养反硝化作为生物脱氮技术中的两种方法，在去除水体氮素方面受到越来越多的关注。本研究考察了磷对自养和混合营养反硝化反应器脱氮性能和细菌群落结构的影响。通过活性试验评估了自养和混合营养污泥在添加磷前后脱氮活性的变化。高通量测序用于分析细菌群落结构的变化。结果表明，自养和混合营养反硝化过程的 NO-N 去除效率分别提高了 40%和 35%。自养和混合营养污泥的污泥反硝化活性得到了显著增强。此外，磷的添加可以大大提高自养（从 11.83%增加到 64.31%）和混合营养反硝化污泥（从 13.59%增加到 45.12%）中脱氮菌的比例。总的来说，磷的添加可以显著提高贫磷地表水的自养和混合营养反硝化能力。
