Yasuda Takuji, Miyata Yasuyoshi, Nakamura Yuichiro, Sagara Yuji, Matsuo Tomohiro, Ohba Kojiro, Sakai Hideki
Department of Urology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Department of Urology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
In Vivo. 2018 Jul-Aug;32(4):721-729. doi: 10.21873/invivo.11300.
BACKGROUND/AIM: Smoking is a risk factor for carcinogenesis and progression of urothelial cancer (UC). Green tea polyphenol inhibits these malignant behaviors and suppresses human antigen R (HuR) expression, which is associated with malignant aggressiveness. This study aimed to clarify the anti-cancer effects of green tea based on the smoking status of UC patients.
Three hundred and sixty (260 with bladder cancer, BC and 100 with upper tract UC) patients were divided into three groups based on consumption of green tea: low (<1 cup/day, n=119), middle (1-4 cup/day, n=160), and high (>5 cup/day, n=81). HuR immunoreactivity was evaluated immunohistochemically in formalin-fixed specimens.
In never smokers, multivariate analysis showed that the frequency of green tea consumption was a significant predictor (middle: hazard ratio, HR, 0.36, p=0.002; high: HR, 0.20, p=0.003) of urinary tract recurrence. A high consumption of green tea was associated with low rates of urinary tract recurrence and up-grading in UC patients. In BC, high consumption was associated with a lower risk of up-grading (p=0.011) and up-staging (p=0.041) in recurrent cancer. HuR expression in the high-consumption group was lower (p=0.019) than that in other groups. These significant findings were not detected in ever smokers.
High consumption of green tea suppressed urinary tract recurrence and the risks of up-grading and up-staging by recurrence in never smokers. Our results suggested that HuR expression played important roles in such mechanisms.
背景/目的：吸烟是尿路上皮癌（UC）发生和进展的危险因素。绿茶多酚可抑制这些恶性行为并抑制与恶性侵袭性相关的人类抗原R（HuR）表达。本研究旨在根据UC患者的吸烟状况阐明绿茶的抗癌作用。
360例患者（260例膀胱癌、BC患者和100例上尿路UC患者）根据绿茶饮用量分为三组：低饮用量组（<1杯/天，n = 119）、中饮用量组（1 - 4杯/天，n = 160）和高饮用量组（>5杯/天，n = 81）。在福尔马林固定标本中通过免疫组织化学评估HuR免疫反应性。
在从不吸烟者中，多因素分析显示绿茶饮用量频率是尿路复发的显著预测因素（中饮用量组：风险比，HR，0.36，p = 0.002；高饮用量组：HR，0.20，p = 0.003）。高饮用量绿茶与UC患者较低的尿路复发率和肿瘤分级升高相关。在BC患者中，高饮用量与复发性癌症中较低的分级升高风险（p = 0.011）和分期升高风险（p = 0.041）相关。高饮用量组的HuR表达低于其他组（p = 0.019）。在曾经吸烟者中未发现这些显著结果。
高饮用量绿茶可抑制从不吸烟者的尿路复发以及复发导致的分级升高和分期升高风险。我们的结果表明HuR表达在这些机制中起重要作用。
