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人巨细胞病毒核内衣壳的分离、其蛋白质成分的表征，以及证明B衣壳组装蛋白在非感染性包膜颗粒中也大量存在。

Isolation of human cytomegalovirus intranuclear capsids, characterization of their protein constituents, and demonstration that the B-capsid assembly protein is also abundant in noninfectious enveloped particles.

作者信息

Irmiere A, Gibson W

出版信息

J Virol. 1985 Oct;56(1):277-83. doi: 10.1128/JVI.56.1.277-283.1985.

DOI:10.1128/JVI.56.1.277-283.1985
PMID:2993655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC252522/
Abstract

Two types of intranuclear capsids have been recovered from human cytomegalovirus (HCMV, strain AD169)-infected cells. By analogy with strain Colburn (simian CMV) particles, these have been designated as A- and B-capsids. Both types of capsids are composed of proteins with molecular weights of 153,000 (major capsid protein), 34,000 (minor capsid protein), 28,000, and 11,000 (smallest capsid protein). In addition to these species, B-capsids contain a 36,000-molecular-weight (36K) protein which has been designated as the HCMV "assembly protein," based on its similarities to counterparts in strain Colburn CMV (i.e., 37K protein) and herpes simplex virus (i.e., VP22a/p40/NC-3/ICP35e). Peptide comparisons established that the assembly protein of HCMV B-capsids and the 36K protein that distinguishes HCMV noninfectious enveloped particles from virions are the same, providing direct evidence that noninfectious enveloped particles are enveloped B-capsids.

摘要

已从人巨细胞病毒（HCMV，AD169株）感染的细胞中分离出两种核内衣壳。根据与科尔本株（猿猴巨细胞病毒）颗粒的类比，这些被指定为A-衣壳和B-衣壳。两种衣壳均由分子量为153,000（主要衣壳蛋白）、34,000（次要衣壳蛋白）、28,000和11,000（最小衣壳蛋白）的蛋白质组成。除了这些种类外，B-衣壳还含有一种分子量为36,000（36K）的蛋白质，基于其与科尔本株巨细胞病毒（即37K蛋白）和单纯疱疹病毒（即VP22a/p40/NC-3/ICP35e）中对应物的相似性，该蛋白质被指定为HCMV“装配蛋白”。肽段比较表明，HCMV B-衣壳的装配蛋白与区分HCMV非感染性包膜颗粒与病毒体的36K蛋白相同，这提供了直接证据表明非感染性包膜颗粒是包膜B-衣壳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/f32765c2bffe/jvirol00115-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/be61b0a459e9/jvirol00115-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/b05c1b70e47e/jvirol00115-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/6e9b6ae88d82/jvirol00115-0290-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/f32765c2bffe/jvirol00115-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/be61b0a459e9/jvirol00115-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/b05c1b70e47e/jvirol00115-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/6e9b6ae88d82/jvirol00115-0290-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b74/252522/f32765c2bffe/jvirol00115-0291-a.jpg

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DNA-binding proteins specified by herpesvirus saimiri.猴疱疹病毒指定的DNA结合蛋白。
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