Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan 50250, Israel; The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem 7610001, Israel.
The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 7610001, Israel.
Stem Cell Reports. 2018 Jul 10;11(1):288-302. doi: 10.1016/j.stemcr.2018.05.013. Epub 2018 Jun 21.
Aiming to unravel the top of the mammary epithelial cell hierarchy, a subset of the CD49fCD24 mammary repopulating units (MRUs) was identified by flow cytometry, expressing high levels of CD200 and its receptor CD200R1. These MRU repopulated a larger area of de-epithelized mammary fat pads than the rest of the MRUs, termed MRU. MRU maintained a much lower number of divergently defined, highly expressed genes and pathways that support better cell growth, development, differentiation, and progenitor activity than their MRU counterparts. A defined profile of hierarchically associated genes supporting a single-lineage hypothesis was confirmed by in vitro mammosphere analysis that assembled 114 genes with decreased expression from MRU via MRU toward CD200CD200R1 and CD200R1CD200 cells. About 40% of these genes were shared by a previously published database of upregulated genes in mammary/breast stem cells and may represent the core genes involved in mammary stemness.
为了揭开乳腺上皮细胞层次的顶端，通过流式细胞术鉴定了一小部分 CD49fCD24 乳腺再群体单位 (MRU)，其表达高水平的 CD200 及其受体 CD200R1。这些 MRU 比其余的 MRU 重新填充了更大面积的去上皮化乳腺脂肪垫，称为 MRU。MRU 维持的分化程度较低的基因和途径数量要少得多，这些基因和途径支持更好的细胞生长、发育、分化和祖细胞活性，而不是它们的 MRU 对应物。通过体外乳腺球体分析证实了与单一谱系假说相关的层次相关基因的明确特征，该分析组装了 114 个基因，这些基因的表达从 MRU 经 MRU 降低至 CD200CD200R1 和 CD200R1CD200 细胞。这些基因中有大约 40%与之前发表的乳腺/乳房干细胞中上调基因的数据库共享，可能代表参与乳腺干细胞特性的核心基因。
