Hoorneman E M
J Neurosci Methods. 1985 Jul;14(2):109-16. doi: 10.1016/0165-0270(85)90122-0.
Since no simple method for standardized positioning of very young rats in a stereotaxic device has been described in the literature, a new method for stereotaxic placement in the neonatal rat was developed, which only requires a simple body mould of the rat pup, and a minor adaptation of a rabbit stereotaxic apparatus (Kopf). The costs of the materials needed for construction of the neonatal rat body mould were approximately $1.-. Construction of the body mould took about one hour. Reliability and accuracy of the new method are demonstrated in a series of experiments in which the A10 group of dopaminergic cell bodies in the ventral tegmental area of 1-day-old rats were lesioned bilaterally: 15 out of 20 angular electrode placements resulted in lesions, the centres of which were located within the A10 group of dopaminergic cell bodies in the ventral tegmental area (max. 0.8 mm in diameter).
由于文献中尚未描述在立体定位装置中对非常年幼的大鼠进行标准化定位的简单方法，因此开发了一种用于新生大鼠立体定位放置的新方法，该方法仅需要幼鼠的简单身体模具，以及对兔立体定位仪（ Kopf）进行轻微改造。构建新生大鼠身体模具所需材料的成本约为1美元。构建身体模具大约需要一个小时。在一系列实验中证明了该新方法的可靠性和准确性，在这些实验中，对1日龄大鼠腹侧被盖区的A10多巴胺能细胞体组进行双侧损伤：20次角电极放置中有15次导致损伤，损伤中心位于腹侧被盖区的A10多巴胺能细胞体组内（直径最大0.8毫米）。
