Tejedor-Junco María Teresa, González-Martín Margarita, Bermeo-Garrido Estefanía, Villasana-Loaiza Rebeca, Carretón-Gómez Elena
Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe "Físico" s/n, 35016, Las Palmas de Gran Canaria, Canary Islands, Spain.
Microbiology, Faculty of Veterinary, University of Las Palmas de Gran Canaria, P.O. BOX 550, 35080, Las Palmas de Gran Canaria, Canary Islands, Spain.
Vet Res Commun. 2018 Sep;42(3):227-232. doi: 10.1007/s11259-018-9727-z. Epub 2018 Jun 24.
Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance.
强力霉素是一种抗生素，除了经典的抗菌用途外，还被用于治疗寄生虫病，如犬心丝虫病。尽管人们担心这种抗生素的过度使用可能会降低临床重要细菌的敏感性，但此前从未研究过强力霉素长期治疗对感染心丝虫的犬类的影响。我们分析了这种治疗对金黄色葡萄球菌和肠球菌耐药性的影响。在这项研究中，纳入了17只感染心丝虫的犬（10只已完成强力霉素治疗，7只尚未开始治疗）。从每只犬的两个部位获得了24株金黄色葡萄球菌分离株。治疗后，73.3%的分离株对至少一种抗生素耐药，但治疗前只有22.2%。大多数对强力霉素耐药的分离株来自接受过治疗的犬。在45.6%的分离株中检测到红霉素耐药或中度敏感，其中大多数来自治疗后的犬。对于肠球菌，从粪便样本中获得了48株分离株（治疗前25株，治疗后23株）。治疗前，32%的分离株至少对一种抗生素耐药，而治疗后，这一数据上升至65%。比较治疗前后的分离株，观察到对强力霉素（12%对21.74%）和红霉素（20%对39.13%）的耐药性明显增加。尽管目前的工作是一项初步研究，但结果鼓励开展进一步的研究，以确定强力霉素长期治疗对抗菌药物耐药性的影响。
