Mel'nikov A A, Chernov A P, Fodor I I
Mol Biol (Mosk). 1985 May-Jun;19(3):610-6.
Plasmidphage lambda NM::pBR322 has been constructed in vitro and characterized. Under normal conditions the hybrid DNA molecule undergoes a lytic cycle of phage development, whereas in the presence of antibiotic lambda DNA replicates in the cell extrachromosomally as a plasmid. Properties of plasmidphage lambda NM::pBR322 have been compared with the earlier constructed lambda gt::pMB9. It has been demonstrated that plasmid pMB9 in vivo can be precisely excised from the lambda gt::pMB9.
质粒噬菌体λNM::pBR322已在体外构建并进行了表征。在正常条件下，杂交DNA分子经历噬菌体发育的裂解周期，而在抗生素存在的情况下，λDNA作为质粒在细胞内进行染色体外复制。已将质粒噬菌体λNM::pBR322的特性与早期构建的λgt::pMB9进行了比较。已经证明，体内的质粒pMB9可以从λgt::pMB9中精确切除。
