Alemà S, Casalbore P, Agostini E, Tatò F
Nature. 1985;316(6028):557-9. doi: 10.1038/316557a0.
PC12 rat phaeochromocytoma cells are a model system that can be used to study both neuronal differentiation and the mechanism of action of nerve growth factor (NGF). PC12 cells respond to NGF protein by shifting from a chromaffin-cell-like phenotype to a neurite-bearing sympathetic neurone-like phenotype. Here we present data on the effect of infection of PC12 cells with retroviruses carrying the src oncogene of Rous sarcoma virus. Previous studies have demonstrated that the expression of src severely affects the synthesis and accumulation of differentiated cell products in a variety of cell types. We show that in the PC12 cell system, expression of v-src appears to have an inductive effect on differentiation that resembles the action of a 'physiological' growth factor.
PC12大鼠嗜铬细胞瘤细胞是一种模型系统，可用于研究神经元分化以及神经生长因子（NGF）的作用机制。PC12细胞通过从嗜铬细胞样表型转变为带有神经突的交感神经元样表型来对NGF蛋白作出反应。在此，我们展示了携带劳氏肉瘤病毒src癌基因的逆转录病毒感染PC12细胞的效应数据。先前的研究表明，src的表达会严重影响多种细胞类型中分化细胞产物的合成和积累。我们发现，在PC12细胞系统中，v-src的表达似乎对分化具有诱导作用，类似于“生理性”生长因子的作用。
