1Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal.
2Centro de Química Estrutural-CQE, DEQ, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
J Med Microbiol. 2018 Aug;67(8):1042-1046. doi: 10.1099/jmm.0.000789. Epub 2018 Jun 25.
Multidrug-resistant bacteria pose a major threat to effective antibiotics and alternatives to fight multidrug-resistant pathogens are needed. We synthetized molybdenum oxide (MoO3) nanoparticles (NP) and determined their antibacterial activity against 39 isolates: (i) eight Staphylococcus aureus, including representatives of methicillin-resistant S. aureus epidemic clones; (ii) six enterococci, including vancomycin-resistant isolates; and (iii) 25 Gram-negative isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterobacter cloacae), including extended spectrum beta-lactamases and carbapenemases producers. All isolates showed a MoO3 NP MIC of 700-800 mg l. MoO3 NP produced a clear inhibition zone for S. aureus and all Gram-negative isolates at concentrations ≥25 mg ml and ≥50 mg ml for enterococci. When the NP solutions were adjusted to pH ~7, the biocidal activity was completely abolished. MoO3 NP create an acidic pH and show a universal antimicrobial activity against susceptible and resistant isolates belonging to the most relevant bacterial species responsible for hospital-acquired infections.
多药耐药菌对有效抗生素构成重大威胁，因此需要寻找对抗多药耐药病原体的替代药物。我们合成了氧化钼（MoO3）纳米颗粒（NP），并测定了它们对 39 株分离菌的抗菌活性：（i）8 株金黄色葡萄球菌，包括耐甲氧西林金黄色葡萄球菌流行克隆的代表；（ii）6 株肠球菌，包括万古霉素耐药株；（iii）25 株革兰氏阴性菌分离株（大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌、阴沟肠杆菌），包括产超广谱β-内酰胺酶和碳青霉烯酶的菌株。所有分离株对 MoO3 NP 的 MIC 均为 700-800mg/L。MoO3 NP 在浓度≥25mg/ml 和≥50mg/ml 时，对金黄色葡萄球菌和所有革兰氏阴性分离株均产生明显的抑制圈，对肠球菌也有抑制作用。当 NP 溶液的 pH 值调节到~7 时，杀菌活性完全丧失。MoO3 NP 会产生酸性 pH 值，并对属于引起医院获得性感染的最重要细菌物种的敏感和耐药分离株表现出普遍的抗菌活性。
