Smith D K, Harper J F, Wallace I S
a Department of Biochemistry and Molecular Biology , University of Nevada, Reno , Reno , NV , USA.
b Department of Chemistry , University of Nevada, Reno , Reno , NV , USA.
Plant Signal Behav. 2018;13(5):e1467687. doi: 10.1080/15592324.2018.1467687. Epub 2018 Jun 25.
Putative protein O-fucosyltransferases (POFTs) represent a large family of Glycosyl Transferase family 65 domain-containing proteins in land plants, with at least 39 proposed members in the Arabidopsis thaliana genome alone. We recently identified a member of this family, AtOFT1 (At3g05320), in which loss-of-function mutants display impaired sexual reproduction that was linked to a defective male gamete. Specifically, oft1 mutant pollen tubes are ineffective at penetrating the stigma-style interface leading to a drastic reduction in seed set and a nearly 2000-fold reduction in pollen transmission. Our findings establish that AtOFT1 plays a critical role in pollen tube penetration through the stigma/style in Arabidopsis and further suggest an important role for protein O-glycosylation events that potentially influence pollen tube mechanical strength or the ability to respond to positional guidance cues during the process of tube growth and fertilization.
推定的蛋白质O-岩藻糖基转移酶（POFTs）是陆地植物中一个包含糖基转移酶65结构域的蛋白质大家族，仅拟南芥基因组中就有至少39个假定成员。我们最近鉴定出该家族的一个成员AtOFT1（At3g05320），其功能缺失突变体表现出有性生殖受损，这与雄性配子缺陷有关。具体而言，oft1突变体花粉管在穿透柱头-花柱界面时无效，导致结实率大幅降低，花粉传播减少近2000倍。我们的研究结果表明，AtOFT1在拟南芥花粉管穿透柱头/花柱过程中起关键作用，并进一步表明蛋白质O-糖基化事件在花粉管生长和受精过程中可能影响花粉管机械强度或对位置引导线索作出反应的能力方面具有重要作用。
