Wobbe C R, Dean F, Weissbach L, Hurwitz J
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710-4. doi: 10.1073/pnas.82.17.5710.
Extracts (0.2 M NaCl) of HeLa cells support replication of DNA containing simian virus 40 (SV40) origin in the presence of SV40 large tumor (T) antigen. The reaction leads to the accumulation of high molecular weight products that represent DNA containing one parental strand and one progeny strand as well as duplex molecules that contain both strands derived from the input deoxynucleoside triphosphates. The replication reaction is inhibited by aphidicolin and by camptothecin, two inhibitors known to inhibit eukaryotic DNA replication in vivo.
在猿猴病毒40（SV40）大T抗原存在的情况下，HeLa细胞提取物（0.2M NaCl）能支持含有SV40复制起点的DNA的复制。该反应导致高分子量产物的积累，这些产物代表含有一条亲代链和一条子代链的DNA，以及包含两条均来源于输入脱氧核苷三磷酸的链的双链分子。该复制反应受到阿非迪霉素和喜树碱的抑制，这两种抑制剂已知在体内可抑制真核生物DNA复制。
