实验性三钙矽酸钙水泥诱导修复性牙本质形成。

Experimental tricalcium silicate cement induces reparative dentinogenesis.

机构信息

KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven, Belgium; Wuhan University, School and Hospital of Stomatology, Ministry of Education, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Wuhan, PR China.

KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven, Belgium.

出版信息

Dent Mater. 2018 Sep;34(9):1410-1423. doi: 10.1016/j.dental.2018.06.016. Epub 2018 Jun 22.

DOI:10.1016/j.dental.2018.06.016

PMID:29941352

Abstract

OBJECTIVES

To overcome shortcomings of hydraulic calcium-silicate cements (hCSCs), an experimental tricalcium silicate (TCS) cement, named 'TCS 50', was developed. In vitro research showed that TCS 50 played no negative effect on the viability and proliferation of human dental pulp cells, and it induced cell odontogenic differentiation. The objective was to evaluate the pulpal repair potential of TCS 50 applied onto exposed minipig pulps.

METHODS

Twenty permanent teeth from three minipigs were mechanically exposed and capped using TCS 50; half of the teeth were scheduled for 7-day and the other half for 70-day examination (n=10). Commercial hCSCs ProRoot MTA and TheraCal LC were tested as references (n=8). Tooth discoloration was examined visually. After animal sacrifice, the teeth were scanned using micro-computed tomography; inflammatory response at day 7 and day 70, mineralized tissue formation at day 70 were assessed histologically.

RESULTS

Up to 70 days, TCS 50 induced no discoloration, ProRoot MTA generated gray/black discoloration in all teeth. For TCS 50, 40.0% pulps exhibited a mild/moderate inflammation at day 7. No inflammation was detected and complete reparative dentin with tubular structures was formed in all pulps after 70 days. ProRoot MTA induced a similar response, TheraCal LC generated a less favorable response in terms of initial inflammation and reparative dentin formation; however, these differences were not significant (Chi-square test of independence: p>0.05).

SIGNIFICANCE

TCS 50 induced reparative dentinogenesis in minipig pulps. It can be considered as a promising pulp-capping agent, also for aesthetic areas.

摘要

目的

为了克服水硬硅酸钙水泥（hCSCs）的缺点，开发了一种实验性的硅酸三钙（TCS）水泥，命名为“TCS 50”。体外研究表明，TCS 50 对人牙髓细胞的活力和增殖没有负面影响，并且诱导细胞成牙本质分化。本研究旨在评估应用于暴露的小型猪牙髓的 TCS 50 的牙髓修复潜力。

方法

将三只小型猪的 20 颗恒牙通过机械法暴露并用 TCS 50 覆盖；其中一半的牙齿在 7 天和 70 天进行检查（n=10），另一半则用作对照。商业 hCSCs ProRoot MTA 和 TheraCal LC 也被用作参考（n=8）。通过肉眼检查牙变色情况。动物处死前，使用微计算机断层扫描扫描牙齿；第 7 天和第 70 天评估炎症反应，第 70 天评估矿化组织形成情况。

结果

在 70 天内，TCS 50 没有引起变色，ProRoot MTA 则使所有牙齿都变成了灰色/黑色。在 TCS 50 组中，40.0%的牙髓在第 7 天表现出轻度/中度炎症。70 天后，所有牙髓均未检测到炎症，形成了具有管状结构的完整修复性牙本质。ProRoot MTA 引起了类似的反应，TheraCal LC 在初始炎症和修复性牙本质形成方面的反应较差；然而，这些差异没有统计学意义（独立性卡方检验：p>0.05）。