Tziafa Christina, Koliniotou-Koumpia Eugenia, Papadimitriou Serafim, Tziafas Dimitrios
Department of Operative Dentistry, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Department of Clinical Sciences, Veterinary School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Endod. 2014 Dec;40(12):1967-71. doi: 10.1016/j.joen.2014.07.021. Epub 2014 Oct 11.
The aim of this study was to evaluate pulpal responses after experimental direct pulp capping of mechanically exposed teeth with a new calcium silicate-based dentin replacement material.
Thirty-four anterior and posterior teeth of 3 miniature swine were used. Class V or I cavities were prepared on the buccal or occlusal surfaces, respectively. Pulpal exposures were further performed using a round carbide bur 0.8 mm in diameter. Exposures were treated with white MTA Angelus (Angelus, Londrina, PR, Brazil) or Biodentine (Septodont, Saint Maur des Fosses, France), and the cavities were further restored with Biodentine. The pulpal tissue responses were histologically assessed at postoperative periods of 3 and 8 weeks. Data were statistically analyzed using the Kruskal Wallis and the Mann-Whitney U tests.
Inflammatory infiltration or pulp tissue necrosis was not found in any of the specimens. All teeth showed mineralized matrix formation in the form of a complete hard tissue bridge composed of osteodentin or osteodentin followed by a discontinuous or continuous reparative dentin zone. A significantly higher thickness of the hard tissue bridge was found in the group of teeth treated with Biodentine at both 3 and 8 weeks. A number of teeth, which were under root development at the onset of the experimental procedures, exhibited ectopic pulp calcification.
The application of both calcium silicate-based materials in direct contact with the mechanically exposed pulp of healthy miniature swine teeth led to pulp repair with complete hard tissue bridge formation. The thickness of hard tissue bridges was significantly higher after pulp capping with Biodentine.
本研究的目的是评估用一种新型硅酸钙基牙本质替代材料对机械性露髓牙齿进行实验性直接盖髓术后的牙髓反应。
使用3只小型猪的34颗前牙和后牙。分别在颊面或咬合面制备V类或I类洞。使用直径0.8 mm的圆形硬质合金车针进一步造成露髓。露髓处用白色MTA Angelus（巴西隆德里纳市安热卢斯公司）或Biodentine（法国圣莫尔代福塞市Septodont公司）处理，然后用Biodentine进一步修复窝洞。在术后3周和8周对牙髓组织反应进行组织学评估。数据采用Kruskal Wallis检验和Mann-Whitney U检验进行统计学分析。
所有标本均未发现炎症浸润或牙髓组织坏死。所有牙齿均显示有矿化基质形成，表现为完整的硬组织桥，由骨样牙本质或骨样牙本质组成，随后是间断或连续的修复性牙本质区。在术后3周和8周时，用Biodentine治疗的牙齿组中硬组织桥的厚度明显更高。一些在实验开始时处于牙根发育阶段的牙齿出现了异位牙髓钙化。
两种硅酸钙基材料直接接触健康小型猪牙齿的机械性露髓牙髓后，均能促进牙髓修复并形成完整的硬组织桥。用Biodentine盖髓后硬组织桥的厚度明显更高。
