Department of Pharmacy, Henan Provincial People's Hospital, and People's Hospital of Zhengzhou University, Zhengzhou, China.
Department of Nuclear Medicine, Henan Provincial People's Hospital, and People's Hospital of Zhengzhou University, Zhengzhou, China.
Adv Exp Med Biol. 2018;1068:59-71. doi: 10.1007/978-981-13-0502-3_6.
In the past two decades, super-resolution fluorescence microscopy has undergone a dynamic evolution. Following proof-of-concept studies with stimulated emission depletion (STED) microscopy, several new approaches such as structured illumination microscopy (SIM), photoactivation localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), have been developed for imaging of nanoscale structural details and fast cellular dynamics in biological research. In this chapter, after briefly explaining their principles, we will describe the recent application of these super-resolution techniques in single cell imaging. In addition, the extension of super-resolution microscopy to 3D, multicolor, live-cell imaging and multimodal imaging are also discussed, significantly improving the precision of single cell imaging. Combining with molecular biology, biochemistry and bio-computing algorithms, super-resolution fluorescence microscopy continues to expand its capabilities and provide comprehensive insights into the details of single cells.
在过去的二十年中，超分辨率荧光显微镜经历了动态的发展。在基于受激发射损耗（STED）显微镜的概念验证研究之后，已经开发出了几种新方法，例如结构光照明显微镜（SIM）、光激活定位显微镜（PALM）和随机光学重建显微镜（STORM），用于在生物研究中对纳米级结构细节和快速细胞动力学进行成像。在本章中，在简要解释其原理之后，我们将描述这些超分辨率技术在单细胞成像中的最新应用。此外，还讨论了超分辨率显微镜在 3D、多色、活细胞成像和多模式成像中的扩展，这极大地提高了单细胞成像的精度。通过与分子生物学、生物化学和生物计算算法相结合，超分辨率荧光显微镜不断扩展其功能，并提供对单细胞细节的全面了解。
