Ishibashi M, Fukushima T, Yamaji T
Acta Endocrinol (Copenh). 1985 Aug;109(4):474-80. doi: 10.1530/acta.0.1090474.
The effect of cyproheptadine on growth hormone (GH) and prolactin (Prl) secretion from cultured pituitary adenoma cells of acromegaly and pituitary gigantism was studied. When varying doses of cyproheptadine ranging from 0.01 to 1 microM were added to the incubation media, GH secretion was consistently inhibited and a dose-response relationship was observed between the cyproheptadine concentrations and the amounts of GH released into the media. In pituitary adenomas which concurrently produced and secreted Prl, cyproheptadine likewise suppressed Prl release in a dose-related manner. This effect of cyproheptadine was not blocked by coincubation with serotonin. Similarly, coincubation with a dopaminergic antagonist, haloperidol, failed to reverse the inhibitory action produced by cyproheptadine. When coincubated with dopamine, cyproheptadine further inhibited GH and Prl secretion. These results suggest that cyproheptadine possesses a direct action on human somatotroph adenoma cells to inhibit GH and Prl secretion by an unknown mechanism that is different from serotonergic and dopaminergic systems.
研究了赛庚啶对肢端肥大症和垂体巨人症患者培养的垂体腺瘤细胞生长激素（GH）和催乳素（Prl）分泌的影响。当向孵育培养基中加入0.01至1微摩尔不等的不同剂量赛庚啶时，GH分泌持续受到抑制，并且在赛庚啶浓度与释放到培养基中的GH量之间观察到剂量反应关系。在同时产生和分泌Prl的垂体腺瘤中，赛庚啶同样以剂量相关的方式抑制Prl释放。赛庚啶的这种作用不会因与血清素共同孵育而被阻断。同样，与多巴胺能拮抗剂氟哌啶醇共同孵育也未能逆转赛庚啶产生的抑制作用。当与多巴胺共同孵育时，赛庚啶进一步抑制GH和Prl分泌。这些结果表明，赛庚啶对人促生长激素腺瘤细胞具有直接作用，通过一种不同于血清素能和多巴胺能系统的未知机制抑制GH和Prl分泌。
