Fazekas I, Pásztor E, Slowik F, Bálint K, Dobronyi I, Bácsy E, Gyévai A, Péter F, Blatniczky L, Varga I
National Institute of Neurosurgery, Budapest, Hungary.
Acta Neuropathol. 1993;85(2):167-74. doi: 10.1007/BF00227764.
A pituitary adenoma was transsphenoidally removed from a 4.5-year-old girl suffering from gigantism. Prior to the operation both the growth hormone (GH) and the prolactin (PRL) levels in the serum were elevated. By light microscopy the tumor appeared to be an acidophilic adenoma. Two distinct cell types, the densely granulated and the sparsely granulated cells, could be distinguished by electron microscopy. Double immunolabeling revealed the presence of GH alone in some densely granulated cells and PRL alone in some sparsely granulated cells, as well as GH and PRL co-localized in both of the morphologically distinguished cell types. Both cell types were identified in the monolayer and the suspension cultures by electron microscopy. GH and PRL concentrations in the culture media were measured by radioimmunoassay. The basal secretion of growth hormone was almost uniform during the 3-week cell culture period. GH and PRL release was significantly inhibited by bromocriptine. Our studies revealed a bimorphous and bihormonal mixed adenoma in childhood.
从一名患有巨人症的4.5岁女孩身上经蝶窦切除了垂体腺瘤。手术前血清中生长激素（GH）和催乳素（PRL）水平均升高。光镜下肿瘤表现为嗜酸性腺瘤。通过电子显微镜可区分出两种不同的细胞类型，即密集颗粒细胞和稀疏颗粒细胞。双重免疫标记显示，一些密集颗粒细胞中仅存在GH，一些稀疏颗粒细胞中仅存在PRL，以及在两种形态学上可区分的细胞类型中GH和PRL共定位。通过电子显微镜在单层和悬浮培养物中均鉴定出了这两种细胞类型。通过放射免疫测定法测量培养基中GH和PRL的浓度。在3周的细胞培养期内生长激素的基础分泌几乎是均匀的。溴隐亭显著抑制了GH和PRL的释放。我们的研究揭示了一种儿童期的双形态和双激素混合腺瘤。
