Cytotoxicity, Genotoxicity, Antioxidant Potential and Chemical Composition of Leaves of Campomanesia pubescens (Mart. ex DC.) O.Berg.

BACKGROUND

Plants of the genus Campomanesia belong to the family Myrtaceae and are very abundant in Cerrado areas. Teas from leaves of species of this genus are used for intestinal infections, combating obesity, stomach pathology, fever and among others.

OBJECTIVE

The present study evaluated the chemical composition and antioxidant potential, cytotoxicity and genotoxicity of ethanolic extract from leaves of Campomanesia pubescens (Mart. ex DC.) O.Berg.

METHOD

For the evaluation of antioxidant activity, the free radical DPPH and for determination of phenolic compounds Folin-Ciocalteau reagent were used. Identification of the substances was performed by HPLC-DAD by comparison of the retention times with standards analyzed under the same conditions and by evaluation of molecular absorption spectra in the ultraviolet and visible region. The cytotoxicity, genotoxicity were evaluated using Allium cepa bioassay.

RESULTS

In the ethanolic extract 7-hydroxy-6-methyl-5-methoxyflavanone, 5,7-dihydroxy-6-methylflavanone, 5,7-dihydroxy-8-methylflavanone, 2´,4´-dihydroxy-6´-methoxychalcone, 5,7-dihydroxy-6,8- dimethylflavanone, 2´,4´-dihydroxy-5´-methyl-6´-methoxychalcone and 2´,4´-dihydroxy-3´,5´-dimethyl-6´- methoxychalcone were identified. The extract showed antioxidant activity and cytotoxic effects on cell division and increased chromosomal alterations in Allium cepa test.

CONCLUSION

These results showed antioxidant activity and suggest the cytotoxic and genotoxic effects in Allium cepa of ethanolic extract obtained from the leaves of Campomanesia pubescens.