State Key Laboratory of Tree Genetics and Breeding, Non-timber Forest Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, China.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
J Integr Plant Biol. 2018 Nov;60(11):1070-1082. doi: 10.1111/jipb.12693. Epub 2018 Sep 5.
Increasing evidence indicates that long non-coding RNAs (lncRNAs) play pivotal roles in regulatory networks controlling plant and animal gene expression. However, lncRNA roles in regulating rubber biosynthesis in Eucommia ulmoides, an emerging source of natural rubber (Eu-rubber), are currently unknown. Here, we report on RNA deep-sequencing of E. ulmoides fruits at two developmental stages. Based on application of a stringent pipeline, 29,103 lncRNAs and 9,048 transcripts of uncertain coding potential (TUCPs) were identified. Two differentially expressed (DE) TUCPs appear to simultaneously regulate 12 protein-coding genes involved in Eu-rubber biosynthesis (GIEBs), as well as 95 DE genes. Functional categorization of these 95 DE genes indicated their involvement in subcellular microstructures and cellular processes, such as cell wall, cell division, and growth. These DE genes may participate in the differentiation and development of laticifers, where Eu-rubber is synthesized. A model is proposed in which "commanders" (DE TUCPs) direct the "builders" (DE genes) to construct a "storehouse" of materials needed for Eu-rubber synthesis, and the "workers" (GIEBs) to synthesize Eu-rubber. These findings provide insights into both cis- and trans-polyisoprene biosynthesis in plants, laying the foundation for additional studies of this crucial process.
越来越多的证据表明，长非编码 RNA（lncRNA）在调控动植物基因表达的调控网络中发挥着关键作用。然而，lncRNA 在调节杜仲（Eucommia ulmoides）橡胶生物合成中的作用目前尚不清楚，杜仲是天然橡胶（Eu-rubber）的新兴来源。在这里，我们报告了两个发育阶段杜仲果实的 RNA 深度测序结果。基于严格的管道应用，鉴定出 29103 个 lncRNA 和 9048 个不确定编码潜能（TUCP）转录本。两个差异表达（DE）的 TUCP 似乎同时调节 12 个参与 Eu-rubber 生物合成的蛋白质编码基因（GIEBs）以及 95 个 DE 基因。这些 95 个 DE 基因的功能分类表明它们参与了细胞的亚细胞微结构和细胞过程，如细胞壁、细胞分裂和生长。这些 DE 基因可能参与乳管的分化和发育，Eu-rubber 就是在那里合成的。提出了一个模型，其中“指挥官”（DE TUCPs）指挥“建设者”（DE 基因）构建一个用于 Eu-rubber 合成的物质“仓库”，以及“工人”（GIEBs）来合成 Eu-rubber。这些发现为植物中顺式和反式聚异戊二烯生物合成提供了新的视角，为进一步研究这一关键过程奠定了基础。
