Academy of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
Forestry College, Fujian A&F University, Fuzhou, Fujian, China.
Mol Biol Rep. 2020 Mar;47(3):1979-1990. doi: 10.1007/s11033-020-05294-w. Epub 2020 Feb 10.
Trans-polyisoprene rubber is produced in the tissues of leaves, bark, and fruit of Eucommia ulmoides and is considered an important energy source. Transcript profiles of two tissues from E. ulmoides cv. Qinzhong No. 3, leaf and fruit, were analysed using the Illumina HiSeq 2000 system. In total, 104 million clean reads were obtained and assembled into 58,863 unigenes. Through gene functional classification, 28,091 unigenes (47.72%) were annotated and 65 unigenes have been hypothesized to encode proteins involved in terpenoid biosynthesis. In addition, 10,041 unigenes were detected as differentially expressed unigenes, and 29 of them were putatively related to terpenoid biosynthesis. The synthesis of trans-polyisoprene rubbers in E. ulmoides was hypothesised to be dominated by the mevalonate pathway. Farnesyl diphosphate synthase 2 (FPPS2) was considered a key component in the biosynthesis of trans-polyprenyl diphosphate. Rubber elongation factor 3 (REF3) might be involved in stabilising the membrane of rubber particles in E. ulmoides. To date, 351 simple sequence repeats (SSRs) were validated as polymorphisms from eight E. ulmoides plants (two parent plants and six F individuals), and these could act as molecular markers for genetic map density increase and breeding improvement of E. ulmoides.
杜仲组织中合成反式聚异戊二烯橡胶，被认为是一种重要的能量来源。本研究采用 Illumina HiSeq 2000 系统对杜仲组织（叶片和果实）进行转录组分析。共获得 1.04 亿个清洁读数，组装成 58863 个基因。通过基因功能分类，注释了 28091 个基因（47.72%），并推测其中 65 个基因编码参与萜类生物合成的蛋白质。此外，检测到 10041 个差异表达基因，其中 29 个可能与萜类生物合成有关。杜仲中反式聚异戊二烯橡胶的合成可能主要由甲羟戊酸途径主导。法呢基二磷酸合酶 2（FPPS2）被认为是反式多聚异戊二烯二磷酸生物合成的关键组成部分。橡胶延伸因子 3（REF3）可能参与稳定杜仲橡胶粒子的膜。到目前为止，从 8 株杜仲植物（2 个亲本和 6 个 F 个体）中验证了 351 个简单重复序列（SSR）作为多态性，这些 SSR 可以作为遗传图谱密度增加和杜仲改良的分子标记。
