College of Environmental Science and Engineering , North China Electric Power University , Beijing 102206 , China.
Research Center for Eco-Environmental Sciences , Chinese Academy of Science , Beijing 100085 , China.
Environ Sci Technol. 2018 Jul 17;52(14):7996-8004. doi: 10.1021/acs.est.8b01464. Epub 2018 Jul 6.
Metal oxide nanoparticles can exert adverse effects on humans and aquatic organisms; however, their toxic mechanisms are still unclear. We investigated the toxic effects and mechanisms of copper oxide, zinc oxide, and nickel oxide nanoparticles in Danio rerio using microarray analysis and the comet assay. Copper oxide nanoparticles were more lethal than the other metal oxide nanoparticles. Gene ontology analysis of genes that were differentially expressed following exposure to all three metal oxide nanoparticles showed that the nanoparticles mainly affected nucleic acid metabolism in the nucleus via alterations in nucleic acid binding. KEGG analysis classified the differentially expressed genes to the genotoxicity-related pathways "cell cycle", "Fanconi anemia", "DNA replication", and "homologous recombination". The toxicity of metal oxide nanoparticles may be related to impairments in DNA synthesis and repair, as well as to increased production of reactive oxygen species.
金属氧化物纳米颗粒可能对人类和水生生物产生不良影响，但它们的毒性机制尚不清楚。我们使用微阵列分析和彗星试验研究了氧化铜、氧化锌和氧化镍纳米颗粒对斑马鱼的毒性作用及其机制。氧化铜纳米颗粒比其他金属氧化物纳米颗粒的致死性更强。暴露于这三种金属氧化物纳米颗粒后差异表达基因的基因本体论分析表明，纳米颗粒主要通过改变核酸结合来影响核内核酸代谢。KEGG 分析将差异表达基因分类为与遗传毒性相关的途径，如“细胞周期”、“范可尼贫血”、“DNA 复制”和“同源重组”。金属氧化物纳米颗粒的毒性可能与 DNA 合成和修复受损以及活性氧产生增加有关。
