Wang Jie, Xiong Ai-Zhen, Cheng Rong-Rong, Yang Li, Wang Zheng-Tao, Liu Shao-Yong
The Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(11):2326-2332. doi: 10.19540/j.cnki.cjcmm.20180125.001.
Bear Bile Powder contains bile acids, protein, amino acids, bilirubin and microelements and other compounds. Among them, the bile acids are the most active components. Currently, there are many studies on bile acids, but few reports on other components. Therefore, the purpose of this study is to carry out a systematical analysis of multiple components in drainage Bear Bile Powder from different sources. Bilirubin and protein were quantified by microplate spectrophotometer. The contents of bile acids and amino acids were determined by liquid chromatography coupled with mass spectrometry (LC-MS). The contents of microelements were determined by inductively coupled plasma mass spectrometry (ICP-MS) The result indicated that among 20 batches of bear bile powder from different sources there is high similarity (0.922-0.977). Tauroursodeoxycholic acid (TUDCA) and taurochenodeoxycholic acid (TCDCA) were the two most abundant components. The total contents of them were 41%-59% and met the current standard for quality control of bear bile powder. However, significant differences were found in their contents among samples from different sources. Besides, bilirubin, protein, amino acids and microelements also contributed to the differentiation of samples from different sources. The main components of bear bile powder from the different sources were with satisfactory similarity. But bile acids, bilirubin, protein, amino acids and microelements all contributed to the different among samples. Our present study provided a systematical approach for the better quality control and evaluation of bear bile powder.
熊胆粉含有胆汁酸、蛋白质、氨基酸、胆红素和微量元素等化合物。其中，胆汁酸是最具活性的成分。目前，对胆汁酸的研究较多，但对其他成分的报道较少。因此，本研究的目的是对不同来源引流熊胆粉中的多种成分进行系统分析。胆红素和蛋白质通过酶标仪进行定量。胆汁酸和氨基酸的含量通过液相色谱-质谱联用（LC-MS）测定。微量元素的含量通过电感耦合等离子体质谱（ICP-MS）测定。结果表明，20批不同来源的熊胆粉相似度较高（0.922-0.977）。牛磺熊去氧胆酸（TUDCA）和牛磺鹅去氧胆酸（TCDCA）是含量最高的两种成分。它们的总含量为41%-59%，符合现行熊胆粉质量控制标准。然而，不同来源样品中它们的含量存在显著差异。此外，胆红素、蛋白质、氨基酸和微量元素也导致了不同来源样品的差异。不同来源熊胆粉的主要成分相似度令人满意。但胆汁酸、胆红素、蛋白质、氨基酸和微量元素均导致了样品间的差异。本研究为更好地控制熊胆粉质量和进行评价提供了一种系统方法。
