甲状腺功能亢进和甲状腺功能减退大鼠垂体中的1,25 - 二羟基维生素D3受体

Pituitary 1,25-dihydroxyvitamin D3 receptors in hyperthyroid- and hypothyroid-rats.

作者信息

Kashio Y, Iwasaki J, Chihara K, Kaji H, Kita T, Okimura Y, Fujita T

出版信息

Biochem Biophys Res Commun. 1985 Aug 30;131(1):122-8. doi: 10.1016/0006-291x(85)91779-6.

Abstract

The binding of 1 alpha,25-dihydroxy (26,27-methyl-[3H]) cholecalciferol ([3H]1,25-(OH)2D3) to its receptor in cytosol of the anterior pituitary cells was examined in hyperthyroid- and hypothyroid rats, as well as in normal rats. The binding capacity increased by 41% in L-Thyroxine-treated hyperthyroid rats and decreased by 49% in propylthiouracil-ingested hypothyroid rats as compared with normal control rats, whereas the affinity of the receptor for [3H]-1,25(OH)2D3 showed no difference among these 3 animal groups. These findings indicate that the number of 1,25(OH)2D3 receptors in the pituitary may be regulated by thyroid hormone, and further suggest that 1,25-(OH)2D3 may play some role in regulating functions of the anterior pituitary.

摘要

研究了1α,25 - 二羟基（26,27 - 甲基 - [³H]）胆钙化醇（[³H]1,25 - (OH)₂D₃）与甲状腺功能亢进和甲状腺功能减退大鼠以及正常大鼠垂体前叶细胞胞质中其受体的结合情况。与正常对照大鼠相比，L - 甲状腺素治疗的甲状腺功能亢进大鼠的结合能力增加了41%，丙硫氧嘧啶喂养的甲状腺功能减退大鼠的结合能力降低了49%，而这三组动物中受体对[³H] - 1,25(OH)₂D₃的亲和力没有差异。这些发现表明垂体中1,25(OH)₂D₃受体的数量可能受甲状腺激素调节，进一步提示1,25 - (OH)₂D₃可能在调节垂体前叶功能中发挥一定作用。