Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, No. 9, Section 4, Renmin Nan Road, Chengdu, 610041, Sichuan, People's Republic of China.
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, People's Republic of China.
Appl Biochem Biotechnol. 2019 Jan;187(1):338-351. doi: 10.1007/s12010-018-2824-1. Epub 2018 Jun 27.
To recover a nitrogen resource from high-ammonia-nitrogen wastewater, two amphitrophic hydrogen-oxidizing bacteria (HOB), Paracoccus denitrificans Y5 and P. versutus D6, capable of nitrogen assimilation for single-cell protein (SCP) production were isolated. These two HOB strains could grow autotrophically with H as an electron donor, O as an electron acceptor, CO as a carbon source, and ammonia nitrogen (NH-N) as a nitrogen source. The cell molecular formulas of strains Y5 and D6 determined by autotrophic cultivation were CHON and CHON, respectively. The isolated strains could synchronously remove NH-N and organic carbon and produce SCP via heterotrophic cultivation. The rates of removal of NH-N and soluble chemical oxygen demand reached 35.47 and 49.04%, respectively, for Y5 under mixotrophic cultivation conditions with biogas slurry as a substrate. SCP content of strains Y5 and D6 was 67.34-73.73% based on cell dry weight. Compared with soybean meal, the SCP of Y5 contained a variety of amino acids.
为了从高氨氮废水中回收氮资源，从富含氢气的环境中分离得到两株兼性产氢菌（HOB）：脱氮副球菌（Paracoccus denitrificans）Y5 和施氏假单胞菌（P. versutus）D6，它们能够将氨氮（NH-N）同化用于单细胞蛋白（SCP）的生产。这两株 HOB 菌株可以在以 H2 为电子供体、O2 为电子受体、CO2 为碳源、氨氮（NH-N）为氮源的条件下进行自养生长。通过自养培养确定了菌株 Y5 和 D6 的细胞分子式分别为 CHON 和 CHON。通过异养培养，分离得到的菌株可以同步去除 NH-N 和有机碳，并生产 SCP。在以沼气发酵液为基质的混合营养培养条件下，菌株 Y5 对 NH-N 和可溶性化学需氧量的去除率分别达到 35.47%和 49.04%。菌株 Y5 和 D6 的 SCP 含量占细胞干重的 67.34%-73.73%。与大豆粉相比，Y5 的 SCP 含有多种氨基酸。
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