Isopropanol production from carbon dioxide by using a zero-gap cell with culture broth as catholyte.

Microbial CO fixation is a promising route for sustainable chemical production. The facultative chemolithoautotroph can convert CO into value-added compounds using H and O. Here, we developed a bioelectrochemical system (BES) for isopropanol production that integrates CO fixation with H generation via a zero-gap proton exchange membrane electrolyzer. Using the culture broth as catholyte, a pentlandite-based cathode supplied dissolved H directly to engineered , eliminating the need for pressurized gas. Real-time monitoring of dissolved and off-gas compositions guided system operation, yielding an isopropanol titer of 1.1 g L. The pentlandite electrode remained stable with negligible leaching. This platform demonstrates a scalable approach for coupling electrolysis and microbial synthesis and can be extended to other H-utilizing microbes.