[共价AMP - RNA连接酶复合物的水解稳定性]
[Hydrolytic stability of a covalent AMP-RNA ligase complex].
作者信息
Iuodka B A, Markutskas A Ia
出版信息
Bioorg Khim. 1985 Jun;11(6):821-5.
PMID:2994684
Abstract
Behavior of the covalent [32P]- and [14C]AMP-RNA ligase complex under various conditions has been studied. The covalent structure is shown to be readily cleaved by acid and hydroxylamine and relatively stable to alkali and snake venom phosphodiesterase. Products of degradation of the AMP-RNA ligase and AMP-DNA ligase complexes were compared. The data obtained support the earlier assumption of a phosphoamide bond in the AMP-RNA ligase compound.
摘要
研究了共价[32P]-和[14C]AMP-RNA连接酶复合物在各种条件下的行为。结果表明，其共价结构易被酸和羟胺裂解，而对碱和蛇毒磷酸二酯酶相对稳定。比较了AMP-RNA连接酶和AMP-DNA连接酶复合物的降解产物。所得数据支持了早期关于AMP-RNA连接酶化合物中存在磷酰胺键的假设。
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