Radiation Biology Department, German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Cologne, Germany.
Goodyear Dunlop Tires Operations SA, Colmar Berg, Luxembourg.
Biometals. 2018 Oct;31(5):759-770. doi: 10.1007/s10534-018-0119-1. Epub 2018 Jun 27.
Several Escherichia coli deletion mutants of the Keio collection were selected for analysis to better understand which genes may play a key role in copper or silver homeostasis. Each of the selected E. coli mutants had a deletion of a single gene predicted to encode proteins for homologous recombination or contained functions directly linked to copper or silver transport or transformation. The survival of these strains on pure copper surfaces, stainless steel, and alloys of aluminum, copper and/or silver was investigated. When exposed to pure copper surfaces, E. coli ΔcueO was the most sensitive, whereas E. coli ΔcopA was the most resistant amongst the different strains tested. However, we observed a different trend in sensitivities in E. coli strains upon exposure to alloys of the system Al-Ag-Cu. While minor antimicrobial effects were detected after exposure of E. coli ΔcopA and E. coli ΔrecA to Al-Ag alloys, no effect was detected after exposure to Al-Cu alloys. The release of copper ions and cell-associated copper ion concentrations were determined for E. coli ΔcopA and the wild-type E. coli after exposure to pure copper surfaces. Altogether, compared to binary alloys, ternary eutectic alloys (Al-Ag-Cu) had the highest antimicrobial effect and thus, warrant further investigation.
为了更好地了解哪些基因可能在铜或银稳态中起关键作用，从 Keio 集合中选择了几种大肠杆菌缺失突变体进行分析。所选的每种大肠杆菌突变体都缺失了一个单一的基因，该基因预测编码同源重组蛋白，或者具有直接与铜或银转运或转化相关的功能。研究了这些菌株在纯铜表面、不锈钢和铝、铜和/或银合金上的生存能力。当暴露于纯铜表面时，大肠杆菌 ΔcueO 是最敏感的，而在测试的不同菌株中，大肠杆菌 ΔcopA 是最具抗性的。然而，我们观察到在暴露于 Al-Ag-Cu 系统的合金时，大肠杆菌菌株的敏感性呈现出不同的趋势。虽然在暴露于 Al-Ag 合金后，大肠杆菌 ΔcopA 和大肠杆菌 ΔrecA 检测到了轻微的抗菌作用，但在暴露于 Al-Cu 合金后，没有检测到任何作用。在暴露于纯铜表面后，测定了大肠杆菌 ΔcopA 和野生型大肠杆菌的铜离子释放和细胞相关铜离子浓度。总的来说，与二元合金相比，三元共晶合金（Al-Ag-Cu）具有最高的抗菌效果，因此值得进一步研究。
