玛瑙粉尘的体内肺脏反应及体外细胞毒性和溶血作用

Br J Ind Med. 1985 Oct;42(10):700-6. doi: 10.1136/oem.42.10.700.

The pulmonary response to agate dust in vivo was investigated and also its cytotoxic action on peritoneal macrophages and sheep erythrocytes in vitro. The results were compared with quartz dust as the known fibrogenic dust and emery dust (Corundum) was used as a control dust. Agate increased the wet and dry weight of lungs and induced increased collagen formation and a non-reversible fibrotic reaction in the lungs. The tissue response and lung changes were of milder intensity than seen in rats exposed to quartz. In vitro, the extent of dye-uptake and haemolysis yielded results similar to those of the in vivo studies. Release of lactic dehydrogenase into the culture medium was similar in both the agate and emery exposed cells but significantly less when compared with quartz treated cells.

研究了玛瑙粉尘在体内的肺部反应，以及其在体外对腹腔巨噬细胞和绵羊红细胞的细胞毒性作用。将结果与已知的致纤维化粉尘石英粉尘进行比较，并使用金刚砂粉尘（刚玉）作为对照粉尘。玛瑙增加了肺的湿重和干重，并导致肺中胶原蛋白形成增加和不可逆的纤维化反应。组织反应和肺部变化的强度比暴露于石英的大鼠中观察到的要轻。在体外，染料摄取和溶血程度产生的结果与体内研究相似。乳酸脱氢酶释放到培养基中的情况在暴露于玛瑙和金刚砂的细胞中相似，但与经石英处理的细胞相比明显较少。