Université de Strasbourg, CNRS, Institut Charles Sadron, UPR022, 23 rue du Loess, 67034 Strasbourg Cedex, France.
Soft Matter. 2018 Jul 18;14(28):5800-5810. doi: 10.1039/c8sm01013g.
Cyclodextrins are cyclic oligosaccharides capable of forming inclusion complexes with a variety of molecules, and as such have been recognized as a pharmaceutical and biotechnological asset. Cyclodextrins are known to interact with the components of cell membranes, and this correlates with a significant degree of cytotoxicity. In this work, we report on the mechanism of degradation of a model dioleoyl-phosphatidylcholine (DOPC) bilayer exposed to a solution with increasing concentrations of α-cyclodextrins. By combining optical fluorescence microscopy and quartz-crystal microbalance experiments, we study the evolution of supported lipid bilayers (SLBs) and giant unilamellar vesicles (GUVs). The rate of lipid removal is found to display a strong nonlinear dependence on the cyclodextrin concentration. A mechanism involving lipid aggregates is proposed.
环糊精是具有形成与各种分子的包合复合物的能力的环状低聚糖，因此已被认为是一种药物和生物技术资产。已知环糊精与细胞膜的成分相互作用，这与显著程度的细胞毒性相关。在这项工作中，我们报告了暴露于含有逐渐增加浓度的α-环糊精的溶液中的模型二油酰基-磷脂酰胆碱（DOPC）双层的降解机制。通过结合光学荧光显微镜和石英晶体微天平实验，我们研究了支撑脂质双层（SLB）和巨大的单分子层囊泡（GUV）的演变。发现脂质去除的速率对环糊精浓度表现出强烈的非线性依赖性。提出了一种涉及脂质聚集体的机制。
