Zalitis G M, Kublis G G, Antsans Iu E
Biokhimiia. 1985 Jul;50(7):1083-9.
The binding of angiotensin II and its analogues (13) to rabbit antibodies and glomerular cell receptors from rat adrenal cortex was studied, using the radioimmunoassay method and radioreceptor analysis. Double modifications introduced into the angiotensin structure were found to increase in an additive fashion its binding to the antibodies and renal cell receptors. The relative binding activity of the analogues carrying a double modification can be assessed if the activities of the analogues with the appropriate single modifications are known. It was concluded that the testing of modifications in the peptide structure for their additivity may provide some insight into the conformational properties of peptides during their binding to the protein.
利用放射免疫分析法和放射受体分析法，研究了血管紧张素II及其类似物（13）与兔抗体以及大鼠肾上腺皮质肾小球细胞受体的结合情况。发现对血管紧张素结构进行的双重修饰以累加方式增加了其与抗体和肾细胞受体的结合。如果已知具有适当单一修饰的类似物的活性，就可以评估携带双重修饰的类似物的相对结合活性。得出的结论是，测试肽结构修饰的累加性可能有助于深入了解肽在与蛋白质结合过程中的构象特性。
