Sherman W R, Ackermann K E, Bateman R H, Green B N, Lewis I
Biomed Mass Spectrom. 1985 Aug;12(8):409-13. doi: 10.1002/bms.1200120810.
Fast atom bombardment is shown to produce useful spectra of the three phosphoinositides and the metabolically related phospholipids, lysophosphatidylinositol and phosphatidic acid. Analysis of the [M-H]- ions for fatty ester composition by mass-analysed ion kinetic energy spectra (MIKES) is shown to be inadequate to resolve fatty acyl daughter ions when the parent ion contains isobaric species. However, analysis on a triple sector instrument with and without collisional activation does provide complete compositional information. Quantitative analysis of the fatty ester content of each lipid molecular species is complicated by dissimilar ion yields from fatty acyl-bearing fragments from compositionally different parent ions.
快速原子轰击法可用于产生三种磷酸肌醇以及与代谢相关的磷脂（溶血磷脂酰肌醇和磷脂酸）的有用光谱。通过质量分析离子动能谱（MIKES）对[M-H]-离子的脂肪酸酯组成进行分析时，当母离子包含等压物质时，不足以解析脂肪酰基子离子。然而，在有和没有碰撞活化的三重扇形仪器上进行分析确实能提供完整的组成信息。由于来自组成不同的母离子的含脂肪酰基片段的离子产率不同，使得对每种脂质分子物种的脂肪酸酯含量进行定量分析变得复杂。
