视网膜γ-氨基丁酸神经元：利用抗兔脑谷氨酸脱羧酶抗血清在脊椎动物物种中的定位。

Retinal GABA neurons: localization in vertebrate species using an antiserum to rabbit brain glutamate decarboxylase.

作者信息

Brandon C

出版信息

Brain Res. 1985 Oct 7;344(2):286-95. doi: 10.1016/0006-8993(85)90806-6.

Abstract

Retinal gamma-aminobutyric acid (GABA) neurons have been localized immunocytochemically using a new antiserum against rabbit brain glutamate decarboxylase (GAD). The animals examined were: dogfish, ratfish, goldfish, catfish, turtle, chick, mouse, rat, pig, rabbit, cat and New World monkey. GAD-containing processes, observed as punctate deposits of immunochemical reaction product, formed discrete bands within the inner plexiform layers of all retinas examined. Immunoreactive, and therefore presumably GABAergic, amacrine cells were observed in all species. Displaced GABAergic amacrine cells were observed in the retinas of goldfish, catfish, turtle and chick, and sparsely in the rabbit as well. GABAergic horizontal cells were detected in catfish, goldfish, chick and turtle. Interplexiform cells in the cat and the rat were clearly immunoreactive for glutamate decarboxylase; this is the first report of GABAergic interplexiform cells in the rat.

摘要

利用一种针对兔脑谷氨酸脱羧酶（GAD）的新型抗血清，通过免疫细胞化学方法对视网膜γ-氨基丁酸（GABA）神经元进行了定位。所检查的动物有：角鲨、银鲛、金鱼、鲶鱼、乌龟、鸡、小鼠、大鼠、猪、兔、猫和新大陆猴。含GAD的突起表现为免疫化学反应产物的点状沉积，在所检查的所有视网膜的内网状层内形成离散的条带。在所有物种中均观察到免疫反应性的、因此推测为GABA能的无长突细胞。在金鱼、鲶鱼、乌龟和鸡的视网膜中观察到移位的GABA能无长突细胞，在兔视网膜中也有少量发现。在鲶鱼、金鱼、鸡和乌龟中检测到GABA能水平细胞。猫和大鼠的网间细胞对谷氨酸脱羧酶有明显的免疫反应性；这是关于大鼠GABA能网间细胞的首次报道。