College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Probiotics Antimicrob Proteins. 2019 Sep;11(3):946-956. doi: 10.1007/s12602-018-9434-7.
The effects of Enterococcus faecium on growth, intestinal barrier function, and immune response in Escherichia coli O78-challenged broiler chickens were investigated. Three hundred eight 1-day-old Ross male chickens were randomly assigned into three treatment groups: negative control (C), E. coli O78-infected positive (EP), and E. coli O78-infected with 200 mg/kg E. faecium dietary supplementation (EF). E. faecium significantly increased the body weight on day 10 (P < 0.05) and day 15. Furthermore, these birds had a greater average daily gain compared with the other groups during days 1-10 (P < 0.05). The death rate of the EF chickens dramatically declined. E. faecium supplementation improved the jejunal villus height and the ratio of villus height to crypt depth (P < 0.05) 3 and 7 days post-infection. The mRNA expression of claudin-1 significantly increased by E. faecium (P < 0.05) 3 and 7 days post-infection, and Mucin2 was markedly enhanced (P < 0.05) 3 days post-infection. E. faecium upregulated the mRNA expression of PPAR-γ and IL-10 (P < 0.05) and downregulated that of NF-κB, TLR4, and IL-1β (P < 0.05) in the spleen 3 and 7 days post-infection. Lipopolysaccharide stimulation index was markedly enhanced in the EF group (P < 0.05) 3 days post-infection. The increased liver E. coli number caused by the E. coli O78 challenge was significantly reversed by E. faecium (P < 0.05). E. faecium improved growth and reduced the death rate by regulating the immune response and maintaining the intestinal integrity in E. coli O78-challenged broiler chickens.
研究了屎肠球菌对大肠杆菌 O78 感染肉鸡生长、肠道屏障功能和免疫反应的影响。将 308 只 1 日龄 Ross 雄性鸡随机分为 3 个处理组：阴性对照组（C）、大肠杆菌 O78 感染阳性组（EP）和大肠杆菌 O78 感染 200mg/kg 屎肠球菌日粮添加组（EF）。屎肠球菌显著增加了第 10 天（P<0.05）和第 15 天的体重。此外，与其他组相比，这些鸡在第 1-10 天的平均日增重更大（P<0.05）。EF 鸡的死亡率显著下降。屎肠球菌补充剂改善了感染后第 3 天和第 7 天的空肠绒毛高度和绒毛高度与隐窝深度的比值（P<0.05）。感染后第 3 天和第 7 天，屎肠球菌显著增加了 Claudin-1 的 mRNA 表达（P<0.05），Mucin2 明显增强（P<0.05）。感染后第 3 天，屎肠球菌上调了脾组织中 PPAR-γ 和 IL-10 的 mRNA 表达（P<0.05），下调了 NF-κB、TLR4 和 IL-1β 的 mRNA 表达（P<0.05）。感染后第 3 天，EF 组的脂多糖刺激指数显著增强（P<0.05）。大肠杆菌 O78 感染引起的肝脏大肠杆菌数量增加被屎肠球菌显著逆转（P<0.05）。屎肠球菌通过调节免疫反应和维持肠道完整性，改善了大肠杆菌 O78 感染肉鸡的生长，降低了死亡率。
