低钙后处理状态下小鼠肾上腺皮质肿瘤细胞Y-1的细胞结构与功能

Cellular structure and function of mouse adrenocortical tumor cells Y-1 in the post-treatment state of low Ca2+.

作者信息

Sugihara H, Yonemitsu N, Yun K, Miyabara S

出版信息

Cell Struct Funct. 1985 Sep;10(3):295-303. doi: 10.1247/csf.10.295.

DOI:10.1247/csf.10.295

Abstract

Under lowered Ca2+ content and in the post-treatment state of low Ca2+, we studied the cellular structure and functioning in mouse adrenocortical tumor cells, Y-1. These cells had been maintained in Ham F12 medium containing 10% fetal calf serum. The Ca2+ present in this complete medium was 0.39 mM. Under a slightly lowered Ca2+ (0.29 mM) produced by EGTA, the cells had many blebs on their surfaces and specific functional activity decreased in steroidogenesis as did ACTH reactivity. In the post-treatment state of a low Ca2+, the cellular surface was covered with many short microvilli and there was greater cellular activity than in the control cells. When the Ca2+ concentration was below 0.17 mM, the cellular structure and functioning were disturbed, and there was no recovery even at the physiological Ca2+ after the removal of EGTA.

摘要

在钙离子含量降低的情况下以及处于低钙的后处理状态时，我们研究了小鼠肾上腺皮质肿瘤细胞Y-1的细胞结构和功能。这些细胞一直培养在含有10%胎牛血清的Ham F12培养基中。这种完全培养基中的钙离子浓度为0.39 mM。在由乙二醇双四乙酸（EGTA）导致的钙离子浓度略微降低（0.29 mM）的情况下，细胞表面出现许多泡状突起，类固醇生成的特定功能活性降低，促肾上腺皮质激素（ACTH）反应性也降低。在低钙的后处理状态下，细胞表面覆盖着许多短微绒毛，并且细胞活性比对照细胞更高。当钙离子浓度低于0.17 mM时，细胞结构和功能受到干扰，即使在去除EGTA后恢复到生理钙离子浓度，细胞也无法恢复。