Dermatological Department, Jiangyin Hospital Affiliated to Nanjing University of TCM, Jiangyin, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):4000-4008. doi: 10.26355/eurrev_201806_15285.
Plenty of plant extracts have been used for treating hair loss. This study aims to investigate the effects of liposterolic extracts of Serenoa repens (LSESr) on hair cell growth and regeneration of hair, and clarify the associated mechanisms.
Human keratinocyte cells (HACAT) were cultured, incubated with dihydrotestosterone (DHT) and treated with LSESr. Cell viability was examined by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H- tetrazolium bromide (MTT) assay. Hair loss C57BL/6 mouse model was established by inducing with DHT. Hair growth, density, and thickness were evaluated. Back skin samples were collected and stained with hematoxylin and eosin (HE) assay. B-cell lymphoma-2 (Bcl-2), Bcl-2 associated protein X (Bax), cleaved caspase 3 and transforming growth factor β2 (TGF-β2) were examined using Western blot assay.
LSESr treatment significantly increased HACAT cell viabilities compared to DHT-only treated cells (p<0.05). LSESr treatment post injection of DHT significantly converted skin color from pink to gray and increased hair density, weight and thickness compared to DHT-only treated mice (p<0.05). LSESr treatment significantly triggered follicle growth and decreased inflammatory response. LSESr treatment significantly decreased TGF-β2 and cleaved caspase 3 expression of hair loss mouse models compared to that of DHT treated mice (p<0.05). LSESr treatment significantly enhanced Bcl-2 expression and reduced Bax expression compared to that of DHT treated mice (p<0.05). Meanwhile, effects of LSESr were substantial even achieving to the potential of finasteride.
LSESr promoted the hair regeneration and repair of hair loss mouse models by activating TGF-β signaling and mitochondrial signaling pathway.
许多植物提取物已被用于治疗脱发。本研究旨在探讨 Serenoa repens (LSESr) 的脂溶性提取物对毛细胞生长和毛发生长的影响，并阐明其相关机制。
培养人角质形成细胞（HACAT），用二氢睾酮（DHT）孵育并用 LSESr 处理。使用 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐（MTT）测定法检查细胞活力。通过用 DHT 诱导建立脱发 C57BL/6 小鼠模型。评估毛发生长、密度和厚度。采集背部皮肤样本，用苏木精和伊红（HE）染色法染色。使用 Western blot 测定法检查 B 细胞淋巴瘤-2 (Bcl-2)、Bcl-2 相关蛋白 X (Bax)、裂解的半胱天冬酶 3 和转化生长因子 β2 (TGF-β2)。
与仅用 DHT 处理的细胞相比，LSESr 处理显着增加了 HACAT 细胞活力（p<0.05）。与仅用 DHT 处理的小鼠相比，LSESr 处理后在注射 DHT 时皮肤颜色从粉红色变为灰色，并且毛发密度、重量和厚度增加（p<0.05）。LSESr 处理显着触发了毛囊生长并减少了炎症反应。与 DHT 处理的小鼠相比，LSESr 处理显着降低了脱发小鼠模型中 TGF-β2 和裂解的半胱天冬酶 3 的表达（p<0.05）。与 DHT 处理的小鼠相比，LSESr 处理显着增强了 Bcl-2 的表达并降低了 Bax 的表达（p<0.05）。同时，即使达到非那雄胺的潜力，LSESr 的作用也很显著。
LSESr 通过激活 TGF-β 信号通路和线粒体信号通路促进脱发小鼠模型的毛发生长和修复。
