Liang Huiqing, Yang Jiaen, Tang Jinmo, Wu Chuncheng, Li Hongshan, Chen Shaodong
J Tradit Chin Med. 2016 Oct;36(5):683-8. doi: 10.1016/s0254-6272(16)30090-5.
To investigate the optimal dosage ratio of chlorogenic acid and gardenia glycosides in treating the rates with fatty liver disease induced by high-fat feed.
A rat model of non-alcoholic fatty liver disease (NAFLD) was established by using a high-fat diet. According to mathematical model "uniform design", varying doses of chlorogenic acid and gardenia glycosides have been combined to form 6 medications for the treatment of NAFLD. Samples were then taken to observe pathological changes of the liver tissue (HE staining); changes in the fat metabolism pathway e.g. triglyceride (TG) and free fatty acid (FFA) content; alterations in liver function, i.e. serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity; and differences in Malondialdehyde (MDA) and superoxide dismutase (SOD) content in the liver tissue. Multiple regression analysis was conducted to test the optimal dosage ratio of chlorogenic acid and gardenia glycosides.
Fatty degeneration and vacuole-like changes of different degrees occurred in hepatic cells of the model group. Markers for fat metabolism, serum ALT and AST activities, and expression of MDA in liver tissue significantly increased, while SOD decreased. Combination of 90 mg chlorogenic acid and 90 mg Gardenia glycosides was the optimal dosage ratio of chlorogenic acid and gardenia glycosides in the treatment of rats with fatty liver induced by high-fat diet.
Chlorogenic acid of 90 mg plus gardenia glycosides of 90 mg was the best combination in the treatment of fatty liver disease in rats induced by high-fat feed.
探讨绿原酸与栀子苷治疗高脂饲料诱导的大鼠脂肪肝的最佳剂量配比。
采用高脂饮食建立大鼠非酒精性脂肪性肝病（NAFLD）模型。根据“均匀设计”数学模型，将不同剂量的绿原酸和栀子苷组合，形成6种治疗NAFLD的药物。然后采集样本，观察肝组织病理变化（苏木精-伊红染色）；脂肪代谢途径变化，如甘油三酯（TG）和游离脂肪酸（FFA）含量；肝功能改变，即血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性；以及肝组织中丙二醛（MDA）和超氧化物歧化酶（SOD）含量差异。进行多元回归分析以检验绿原酸和栀子苷的最佳剂量配比。
模型组肝细胞出现不同程度的脂肪变性和空泡样改变。脂肪代谢指标、血清ALT和AST活性以及肝组织中MDA表达显著升高，而SOD降低。90 mg绿原酸与90 mg栀子苷联合使用是治疗高脂饮食诱导的大鼠脂肪肝时绿原酸和栀子苷的最佳剂量配比。
90 mg绿原酸加90 mg栀子苷是治疗高脂饲料诱导的大鼠脂肪肝的最佳组合。
