Jović Marko, Nikolić Ivan, Todorović Vera, Petrović Aleksandar, Petrović Vladimir, Mojsilović Marijola, Denčić Tijana
Department of Histology and Embryology, Faculty of Medicine, University of Nis, Nis, Serbia.
Department of Histology and Embryology, Faculty of Stomatology Pančevo, University of Business Academy, Novi Sad, Serbia.
Cells Tissues Organs. 2018;205(3):164-177. doi: 10.1159/000489476. Epub 2018 Jun 27.
In the absence of systematized data on the extracellular matrix components during prenatal liver development, the present study aimed to investigate the time of appearance and distribution of collagen types I, III, and IV and laminin. The study material included embryonic and fetal livers, aged 7-37 weeks, categorized into 3 trimesters. The material was stained using hematoxylin-eosin and immunohistochemistry methods for the identification of collagen I, III, and IV and laminin. Collagen I was detected near the end of the first trimester in the capsules and walls of interlobular veins. As the liver matures, collagen I is increasingly abundant in the capsules, portal area connective tissues, arterial walls, interlobular veins, sinusoids, and central veins. Collagen III and collagen IV appear in the middle of the first trimester in the capsules, portal areas, and walls of central veins, as well as the sinusoids particularly. In trimesters 2 and 3, these collagens are increasingly present in all the structures, but collagen IV is also present in nerve fibers. Laminin is sporadically present adjacent to the sinusoids in trimester 1, while in trimesters 2 and 3 this protein commonly appears in the walls of arteries and interlobular veins, in the basal membrane of bile ducts, and in nerve fibers. The contents of collagen I, III, and IV increase during prenatal development in the liver capsule, arterial and vein walls, sinusoids, and portal area. Laminin expression is consistent with that of the collagens with the exception that, within lobules, laminin disappears with liver maturation.
由于缺乏关于产前肝脏发育过程中细胞外基质成分的系统数据，本研究旨在调查I型、III型和IV型胶原蛋白以及层粘连蛋白的出现时间和分布情况。研究材料包括7至37周龄的胚胎和胎儿肝脏，分为三个孕期。使用苏木精-伊红染色和免疫组织化学方法对材料进行染色，以鉴定I型、III型和IV型胶原蛋白以及层粘连蛋白。I型胶原蛋白在孕早期接近尾声时在小叶间静脉的包膜和壁中被检测到。随着肝脏成熟，I型胶原蛋白在包膜、门静脉区结缔组织、动脉壁、小叶间静脉、肝血窦和中央静脉中越来越丰富。III型胶原蛋白和IV型胶原蛋白在孕早期中期出现在包膜、门静脉区和中央静脉壁以及特别是肝血窦中。在孕中期和孕晚期，这些胶原蛋白在所有结构中越来越多，但IV型胶原蛋白也存在于神经纤维中。层粘连蛋白在孕早期偶尔出现在肝血窦附近，而在孕中期和孕晚期，这种蛋白质通常出现在动脉壁和小叶间静脉壁、胆管基底膜以及神经纤维中。I型、III型和IV型胶原蛋白的含量在肝脏包膜、动脉和静脉壁、肝血窦和门静脉区的产前发育过程中增加。层粘连蛋白的表达与胶原蛋白一致，不同之处在于，在小叶内，层粘连蛋白随着肝脏成熟而消失。
