Cai Xiangdong, Wang Bo, Nian Linge, Cheng Tao, Zhang Chunxia, Li Lu, Zhang Guangrui, Xiao Jianxi
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.
School of Life Sciences, Lanzhou University, Lanzhou, 730000, PR China.
Mater Today Bio. 2024 Mar 14;26:101026. doi: 10.1016/j.mtbio.2024.101026. eCollection 2024 Jun.
The accurate detection of multiplex collagen biomarkers is vital for diagnosing and treating various critical diseases such as tumors and fibrosis. Despite the attractive optical properties of quantum dots (QDs), it remains technically challenging to create stable and specific QDs-based probes for multiplex biological imaging. We report for the first time the construction of multi-color QDs-based peptide probes for the simultaneous fingerprinting of multiplex collagen biomarkers in connective tissues. A bipeptide system composed of a glutathione (GSH) host peptide and a collagen-targeting guest peptide (CTP) has been developed, yielding CTP-QDs probes that exhibit exceptional luminescence stability when exposed to ultraviolet irradiation and mildly acidic conditions. The versatile bipeptide system allows for facile one-pot synthesis of high-quality multicolor CTP-QDs probes, exhibiting superior selectivity in targeting critical collagen biomarkers including denatured collagen, type I collagen, type II collagen, and type IV collagen. The multicolor CTP-QDs probes have demonstrated remarkable efficacy in simultaneously fingerprinting multiple collagen types in diverse connective tissues, irrespective of their status, whether affected by injury, diseases, or undergoing remodeling processes. The innovative multicolor CTP-QDs probes offer a robust toolkit for the multiplex fingerprinting of the collagen suprafamily, demonstrating significant potential in the diagnosis and treatment of collagen-related diseases.
准确检测多种胶原蛋白生物标志物对于诊断和治疗肿瘤及纤维化等各种重大疾病至关重要。尽管量子点（QDs）具有吸引人的光学特性，但创建用于多重生物成像的稳定且特异性的基于量子点的探针在技术上仍然具有挑战性。我们首次报道了构建基于多色量子点的肽探针，用于在结缔组织中同时对多种胶原蛋白生物标志物进行指纹识别。已开发出一种由谷胱甘肽（GSH）主体肽和胶原蛋白靶向客体肽（CTP）组成的双肽系统，产生的CTP-QDs探针在暴露于紫外线照射和轻度酸性条件下时表现出出色的发光稳定性。这种通用的双肽系统允许简便地一锅合成高质量的多色CTP-QDs探针，在靶向关键胶原蛋白生物标志物（包括变性胶原蛋白、I型胶原蛋白、II型胶原蛋白和IV型胶原蛋白）方面表现出卓越的选择性。多色CTP-QDs探针已证明在同时对不同结缔组织中的多种胶原蛋白类型进行指纹识别方面具有显著功效，无论它们的状态如何，是否受到损伤、疾病影响或正在经历重塑过程。创新的多色CTP-QDs探针为胶原蛋白超家族的多重指纹识别提供了一个强大的工具包，在胶原蛋白相关疾病的诊断和治疗中显示出巨大潜力。
