Meng Lei, Hu Hui-Juan, Shang Tong, Tian Wen-Cang, Zhang Ming-Ming, Li Yong-Jie, Li Li, Wei Sheng-Li
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China.
Beijing Chinese Medicine Hospital Affiliated to Capital Medical University, Beijing 100035, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(12):2495-2502. doi: 10.19540/j.cnki.cjcmm.2018.0079.
Eight kinds of provenance of Rheum palmatum collected from 4 provinces Sichuan, Ningxia, Gansu, Shannxi as test materials, which were transplanted under 3 different environments by using complete randomized block design with three replicates. The contents of the chemical components was determined by HPLC. This study aimed at analyzing the effect of genotype, environment and their interactions on the 4 kinds of functional components (phenolic acids, bianthrone, free anthraquinones and combined anthraquinones) in 14 kinds of active components of R. palmatum, in order to provide a theoretical basis for the selection of cultivated R. palmatum in high quality producing area and excellent provenance. The functional components of R. palmatum were influenced by genotype and environment. The content of phenolic acids was mainly influenced by environment, and the other three kinds of functional components were affected by environment and their interactions. The proportion of environment was larger. The cultivation quality of R. palmatum should give priority to environment, then choose a provenance. Sichuan may be beneficial in accumulation of free anthraquinones in R. palmatum, Gansu may facilitate the binding of combined anthraquinone, phenolic acids and bianthrone content. Preliminary inference based on the content and proportion of efficacy components, P2 could be potential special medicinal germplasm that have function of heat-clearing and detoxifying drugs. P6 could be potential special medicinal germplasm that activate blood circulation to dissipate blood stasis. P7 and P1 could all be potential specialmedicinal germplasms that exist diarrhea attack characters. The results of this study have important guiding significance for the production of rhubarb precision medicinal materials.
以采自四川、宁夏、甘肃、陕西4省的8种掌叶大黄种源为试验材料,采用完全随机区组设计,3次重复,在3种不同环境下进行移栽。采用高效液相色谱法测定化学成分含量。本研究旨在分析基因型、环境及其互作对掌叶大黄14种活性成分中4种功能成分(酚酸、二蒽酮、游离蒽醌和结合蒽醌)的影响,为优质产区栽培掌叶大黄及优良种源的选择提供理论依据。掌叶大黄的功能成分受基因型和环境影响。酚酸含量主要受环境影响,其他3种功能成分受环境及其互作影响,环境所占比例较大。掌叶大黄栽培品质应优先考虑环境,其次选择种源。四川可能有利于掌叶大黄中游离蒽醌的积累,甘肃可能有利于结合蒽醌的结合,酚酸和二蒽酮含量也较高。根据药效成分的含量和比例初步推断,P2可能是具有清热解毒功效的潜在特殊药用种质。P6可能是具有活血化瘀功效的潜在特殊药用种质。P7和P1都可能是具有泻下攻积特性的潜在特殊药用种质。本研究结果对大黄精准药材生产具有重要指导意义。