Hu Zhong-Li, Zhang Feng, Huang Bao-Jun, Pan Shao-Jun
Department of Hematology, The First Affilicated Hospital of Bengbu Medical College, Bengbu 233003, Anhui Province, China.
Department of Hematology, The First Affilicated Hospital of Bengbu Medical College, Bengbu 233003, Anhui Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Jun;26(3):727-732. doi: 10.7534/j.issn.1009-2137.2018.03.016.
To investigate the clinical significance of RUNX1-RUNX1T1 expression level in bone marrow of patients with acute non-M3 myeloid leukemia (AML non-M3), and to understand the biological characteristics of RUNX1-RUNX1T1 positive AML expressing lymphoid antigens CD19, CD56 and its effect on the initially induced remission rate and prognosis.
The expression level of RUNX1-RUNX1T1 in bone marrow of 200 patients with newly diagnosed AML (non-M3) was detected by real-time fluorescent Q-PCR, the expression level of lymphoid antigens was detected by flow cytometry, and the relationship of the initially induced remission rate (CR1) with the overall survival (OS) rate was analyzed, the CR1 and OS differences also were analyzed between CD56 and CD56 patients as well as CD19 and CD17 patients in RUNX1-RUNX1T1 positive patients with AML.
The CD56 patients at the initial diagnosis had lower CR1(P<0.05) in RUNX1-RUNX1T1 positive AML patients, the CR1 of CD19 patients was higher than that in CD19 patients at the initial diagnosis (P<0.05). The OS of CD56 patients was significantly high in comparison with CD56 patients (P<0.05), while the OS between CD19 patients and CD19 patients was not significantly different.
The bone marrow CD56 in RUNX1-RUNX1T1 positive AML patients suggests poor prognosis. The CD19 only correlates with CR1, but does not with OS.
探讨急性非M3型髓系白血病（AML非M3）患者骨髓中RUNX1-RUNX1T1表达水平的临床意义，了解表达淋巴样抗原CD19、CD56的RUNX1-RUNX1T1阳性AML的生物学特性及其对初始诱导缓解率和预后的影响。
采用实时荧光定量PCR检测200例初诊AML（非M3）患者骨髓中RUNX1-RUNX1T1的表达水平，采用流式细胞术检测淋巴样抗原的表达水平，分析初始诱导缓解率（CR1）与总生存率（OS）的关系，同时分析RUNX1-RUNX1T1阳性AML患者中CD56阳性与阴性以及CD19阳性与阴性患者之间的CR1和OS差异。
RUNX1-RUNX1T1阳性AML患者初诊时CD56阳性患者的CR1较低（P<0.05），CD19阳性患者的CR1高于初诊时CD19阴性患者（P<0.05）。CD56阳性患者的OS明显高于CD56阴性患者（P<0.05），而CD19阳性与阴性患者之间的OS差异无统计学意义。
RUNX1-RUNX1T1阳性AML患者骨髓CD56阳性提示预后不良。CD19仅与CR1相关，与OS无关。
