[细胞外基质蛋白Reelin在多发性骨髓瘤患者中的表达及其与预后的关系]
[Expression of Extracellular Matrix Protein Reelin in Patients with Multiple Myeloma and Its Relationship with Prognosis].
作者信息
An Quan-Ming, Liu Yuan-Gen, Lin Liang, Liu Yang, Lu Jin, Ge Qing
机构信息
Department of Immunology, Peking University Health Science Center, Beijing 100101,China.
Peking University People's Hospital & Institute of Hematology, Peking University, Beijing 100044, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Jun;26(3):789-795. doi: 10.7534/j.issn.1009-2137.2018.03.026.
OBJECTIVE
To investigate the expression of extracellular matrix protein Reelin (RELN) and its relationship with the prognosis of patients with multiple myeloma.
METHODS
The mononuclear cells were collected from bone marrows of multiple myeloma patients by Ficoll gradient density centrifugation, the CD138 cells were then purified by flow cytometry, the mRNA level of RELN was detected by real time PCR. The myeloma patients were divided into 2 groups according to the relative expression levels of RELN. Then the relationship of RELN expression level with clinical data was analyzed statistically.
RESULTS
The high expression of RELN significantly correlated with the percentage of CD138 cells, progress-free and total survival, but did not correlate significantly with DS (P>0.05) and ISS stages (P>0.05). The RELN high expression group showed higher levels of serum M protein, uric acid and serum calcium, lower hemoglobin, and more abnormal FISH results (including RB1 deletion, 1q21 amplification, IgH recombination, P53 deletion, D13S319 deletion) than those in RELN low expression group.
CONCLUSION
Reelin correlates with the progression of multiple myeloma.
目的
探讨细胞外基质蛋白Reelin（RELN）的表达及其与多发性骨髓瘤患者预后的关系。
方法
采用Ficoll梯度密度离心法从多发性骨髓瘤患者骨髓中分离单个核细胞，通过流式细胞术纯化CD138细胞，采用实时荧光定量PCR检测RELN的mRNA水平。根据RELN相对表达水平将骨髓瘤患者分为两组，然后对RELN表达水平与临床资料的关系进行统计学分析。
结果
RELN高表达与CD138细胞百分比、无进展生存期和总生存期显著相关，但与DS分期（P>0.05）和ISS分期（P>0.05）无显著相关性。与RELN低表达组相比，RELN高表达组血清M蛋白、尿酸和血钙水平更高，血红蛋白更低，FISH结果异常更多（包括RB1缺失、1q21扩增、IgH重排、P53缺失、D13S319缺失）。
结论
Reelin与多发性骨髓瘤的进展相关。
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